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Low molecular weight analytes in water by spectral interferometry using a competitive immunoassay

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  • Water Analysis
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Abstract

The optical detection principle of reflectometric interference spectroscopy (RIFS) is applied for the first time to the immunological detection of low molecular weight analytes. Dinitrophenol/Anti-Dinitrophenol is used as a model system for pesticide detection. The spectrometric principle allows the sensitive determination of small changes in the thickness of a thin film caused by the reaction of an antigen and its antibody. Changes in the optical thickness correlate with the concentration of the analyte. Time resolved measurements allow the dynamic monitoring of the antigen-antibody-interaction. Detection limits currently achieved are in the ppb-range.

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Authors and Affiliations

  1. Institute for Physical and Theoretical Chemistry, University of Tübingen, Auf der Morgenstelle 8, D-72076, Tübingen, Germany

    G. Lang, A. Brecht & G. Gauglitz

Authors
  1. G. Lang
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  2. A. Brecht
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  3. G. Gauglitz
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Lang, G., Brecht, A. & Gauglitz, G. Low molecular weight analytes in water by spectral interferometry using a competitive immunoassay. Fresenius J Anal Chem 348, 602–605 (1994). https://doi.org/10.1007/BF00323941

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  • DOI: https://doi.org/10.1007/BF00323941

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