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Phenotyping of malignant hematopoietic cells

Analysis of 1200 cases of leukemia-lymphoma

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Summary

1255 cases of leukemia-lymphoma were tested between 1972 and 1984 by multiple marker analysis. Routine leukemia phenotyping was performed using standard morphological and cytochemical techniques in combination with clinical and histo-pathological information; the main emphasis was put on immunological surface marker analysis using erythrocyte rosette assays, TdT and a large panel of poly- and monoclonal antibody tests. The 1255 cases were divided into these major types and subtypes: 349 cases of ALL and related immature T- and Burkitt-lymphomas (cALL, pre B-ALL, B-ALL and Burkitt-lymphomas, T-ALL and immature, mostly leukemic T-lymphomas, Null-ALL), 454 cases of mature T- and B-cell malignancies (T-CLL, mycosis fungoides, Sezary-syndrome, T-lymphomas, B-CLL, hairy cell leukemia, multiple myeloma, B-lymphomas), 263 cases of acute myeloid leukemias (AML, AMMoL/AMoL), 182 cases of chronic myeloid leukemias (CML in chronic phase, CMoL, CML in blast crisis), 6 cases of erythroleukemia and 1 case of megakaryoblastic leukemia. A simplified classification scheme which has been used in our laboratories is presented. Phenotyping is of diagnostic, prognostic and therapeutic relevance, most evidently for patients with ALL. Routine leukemia phenotyping should be performed with highly standardized techniques and reagents and by combining information from several fields in the multiple marker analysis. New areas of leukemia research might become very useful for the routine procedure of phenotyping.

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Abbreviations

ALL:

acute lymphoblastic leukemia

AML:

acute myeloblastic leukemia

AMMoL:

acute myelomonoblastic leukemia

AMoL:

acute monoblastic leukemia

cALL:

common ALL

CLL:

chronic lymphocytic leukemia

CML:

chronic myelocytic leukemia

CML-BC:

CML in blastic crisis

CMoL:

chronic monocytic leukemia

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Author notes

  1. Hans Guenter Drexler

    Present address: Department of Haematology, The Royal Free Hospital, Pond Street, NW3 2QG, Hampstead, London, England

Authors and Affiliations

  1. Stritch School of Medicine, Loyola University of Chicago, 60153, Maywood, Illinois, USA

    Hans Guenter Drexler, Mira Menon, Harry Messmore & Jun Minowada

  2. Hines Veterans Administration Medical Center, 60141, Hines, Illinois, USA

    Hans Guenter Drexler, Mira Menon, Harry Messmore & Jun Minowada

  3. Roswell Park Memorial Institute, 14263, Buffalo, New York, USA

    Kimitaka Sagawa, Eiji Tatsumi, Hirofumi Koshiba, Toshioki Koishi, Keisuke Minato, Tohru Sugimoto, Masaki Saito, Masuji Morita, John L. Pauly, Tin Han, Arnold I. Freeman & Jun Minowada

Authors
  1. Hans Guenter Drexler
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  2. Mira Menon
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  3. Kimitaka Sagawa
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  4. Eiji Tatsumi
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  5. Hirofumi Koshiba
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  6. Toshioki Koishi
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  7. Keisuke Minato
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  8. Tohru Sugimoto
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  9. Masaki Saito
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  10. Masuji Morita
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  11. John L. Pauly
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  12. Tin Han
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  13. Arnold I. Freeman
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  14. Harry Messmore
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  15. Jun Minowada
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Drexler, H.G., Menon, M., Sagawa, K. et al. Phenotyping of malignant hematopoietic cells. Blut 52, 99–109 (1986). https://doi.org/10.1007/BF00321072

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  • DOI: https://doi.org/10.1007/BF00321072

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