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Recent trends in platelet antigen/antibody detection

  • Proceedings Of The International Workshop On ITP
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Summary

The detection of platelet antigens and platelet antibodies has always been difficult. Recent technical achievements are due to the availability of more specific and sensitive reagents (i.e. F(ab)2 fragments; higher specific activity of labels; monoclonal antibodies directed against platelet membrane constituents etc.) and the application of advanced immunological assays to platelet immunology (i.e. blotting assays, “capture”-ELISA's and radioimmunoprecipitation in conjunction with SDS polyacrylamide gel electrophoresis). These techniques have permitted the definition and immunochemical characterization of new platelet allo- and autoantigens, have assisted in clinical diagnosis and promoted our understanding of pathogenic mechanisms. Illustrative examples are presented and discussed.

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Authors and Affiliations

  1. Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University, Langhansstrasse 7, D-6300, Giessen, Germany

    C. Mueller-Eckhardt, V. Kiefel & S. Santoso

Authors
  1. C. Mueller-Eckhardt
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  2. V. Kiefel
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  3. S. Santoso
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Supported by the Deutsche Forschungsgemeinschaft (Mu 277/9-6, 7)

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Mueller-Eckhardt, C., Kiefel, V. & Santoso, S. Recent trends in platelet antigen/antibody detection. Blut 59, 35–43 (1989). https://doi.org/10.1007/BF00320246

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  • DOI: https://doi.org/10.1007/BF00320246

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