Summary
Mapping of monoaminergic systems in the brain of the newt Triturus alpestris was achieved with antisera against (1) thyrosine hydroxylase (TH), (2) formaldehyde-conjugated dopamine (DA), and (3) formaldehyde-conjugated serotonin (5-HT). In the telencephalon, the striatum was densely innervated by a large number of 5-HT-, DA-and TH-immunoreactive (IR) fibers; IR fibers were more scattered in the amygdala, the medial and lateral forebrain bundles, and the anterior commissure. In the anterior and medial diencephalon, TH-IR perikarya contacting the cerebrospinal fluid (CSF-C perikarya) were located in the preoptic recess organ (PRO), the organum vasculosum laminae terminalis and the suprachiasmatic nucleus. Numerous TH-IR perikarya, not contacting the CSF, were present in the posterior preoptic nucleus and the ventral thalamus. At this level, DA-IR CSF-C neurons were only located in the PRO. In the posterior diencephalon, large populations of 5-HT-IR and DA-IR CSF-C perikarya were found in the paraventricular organ (PVO) and the nucleus infundibularis dorsalis (NID); the dorsal part of the NID additionally presented TH-IR CSF-C perikarya. Most regions of the diencephalon showed an intense monoaminergic innervation. In addition, numerous TH-IR, DA-IR and 5-HT-IR fibers, orginating from the anterior and posterior hypothalamic nuclei, extended ventrally and reached the median eminence and the pars intermedia of the pituitary gland. In the midbrain, TH-IR perikarya were located dorsally in the pretectal area. Ventrally, a large group of TH-IR cell bodies and some weakly stained DA-IR and 5-HT-IR neurons were observed in the posterior tuberculum. No dopaminergic system equivalent to the substantia nigra was revealed. The possible significance of the differences in the distribution of TH-IR and DA-IR neurons is discussed, with special reference to the CSF-C neurons.
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Abbreviations
- AM :
-
amygdala
- CAnt :
-
commissura anterior
- CH :
-
commissura hippocampi
- CP :
-
commissura posterior
- Ctm :
-
commissura tecti mesencephali
- DH :
-
dorsal hypothalamus
- DTh :
-
dorsal thalamus
- FLM :
-
fasciculus longitudinalis medialis
- Fsol :
-
fasciculus solitarius
- H :
-
habenula
- LFB :
-
lateral forebrain bundle
- ME :
-
median eminence
- MFB :
-
medial forebrain bundle
- NID :
-
nucleus infundibularis dorsalis
- nIP :
-
neuropil of nucleus interpeduncularis
- NPOP :
-
nucleus preopticus posterior
- NS :
-
nucleus septi
- OVLT :
-
organum vasculosum laminae terminalis
- PD :
-
pars distalis
- Pdo :
-
dorsal pallium
- PHi :
-
primordium hippocampi
- PI :
-
pars intermedia
- Pl :
-
lateral pallium
- PN :
-
pars nervosa
- PRO :
-
preoptic recess organ
- Ptec :
-
pretectal area
- PVO :
-
paraventricular organ
- Ra :
-
nucleus raphe
- Rm :
-
nucleus reticularis medius
- SCO :
-
subcommisural organ
- ST :
-
striatum; strm stria medullaris thalami
- strt :
-
stria terminalis thalami
- TM :
-
tegmentum mesencephali
- TO :
-
tectum opticum
- TP :
-
tuberculum posterius
- trch :
-
tractus cortico-habenularis
- trmp :
-
tractus mamillopeduncularis
- VH :
-
ventral hypothalamus
- Vm :
-
nucleus motorius nervi trigemini
- VTh :
-
ventral thalamus
- II :
-
optic nerve
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Corio, M., Thibault, J. & Peute, J. Distribution of catecholaminergic and serotoninergic systems in forebrain and midbrain of the newt, Triturus alpestris (Urodela). Cell Tissue Res 268, 377–387 (1992). https://doi.org/10.1007/BF00318806
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DOI: https://doi.org/10.1007/BF00318806