Summary
The pattern of [3H]thymidine incorporation in the dorsolateral wall of the embryonic tectum was studied and compared one hour after injection of the label in the rhesus monkey (Macaca mulatta) at stages 11–20 (25–37 days of gestation) and in the C57BL mouse at stages 14–22 (9–14 days of gestation). During the early stages of development, the labeled nuclei were located peripherally in the ventricular zone in both the rhesus monkey and mouse embryo, although a number of labeled nuclei tended to occur closer to the ventricular border in the mouse, whereas there was little or no encroachment, at the ventricular border in the rhesus monkey. The ventricular zone of the rhesus monkey and mouse embryos initially showed a high labeling index (LI) of about 59% which subsequently declined with increasing age However, the decline occurred earlier and more precipitously in the rhesus monkey. At stage 17 of the rhesus monkey the LI had dropped to about 42%, whereas it still remained at 59% in the 12-day mouse, and by stage 20 of the monkey the LI was approximately 26%, in contrast to 41% in the stage 22 (14-day) mouse. At stage 20 of the mouse (12 days of gestation) the intermediate zone became much thicker than in the comparable stage (17) of the rhesus monkey, and this discrepancy continued at each successive stage observed in the current study. Also, whereas lamination became apparent in the intermediate zone of the mouse at stage 22, the monkey tectum at a comparable stage (20) was poorly differentiated.
Similar content being viewed by others
References
Angevine JB (1970) Time of neuron origin in the diencephalon of the mouse. An autoradiographic study. J Comp Neurol139:129–188
Atlas M, Bond VP (1965) The cell generation cycle of the eleven-day mouse embryo. J Cell Biol 26:19–24
Bartelmez GW, Dekaban AS (1962) The early development of the human brain. contr Embryol Carneg Inst 37:13–32
Boulder Committee (1970) Embryonic, vertebrate central nervous system: revised terminology. Anat Rec 166:257–262
Cowan WM (1971) Studies on the development of the avian visual system. In: Pease DC (ed) Cellular aspects of neural growth and differentiation. Univ Calif Press, Los Angeles
Davignon RW, Parker RM, Hendrickx AG (1980) Staging of the early embryonic brain in the baboon (Papio cynocephalus) and rhesus monkey (Macaca mulatta). Anat Embryol 159:317–334
DeLong GR, Sidman RL (1962) Effects of eye removal at birth on histogenesis of the mouse superior colliculus: An autoradiographic analysis with tritiated thymidine. J Comp Neurol 118:205–224
Fujita S, Horii M, Tanimura T, Nishimura H (1964) H3-thymidine autoradiographic studies on cytokinetic responses to X-ray irradiation and to thio-TEPA in the neural tube of mouse embryos. Anat Rec 149:37–48
Gruneberg H (1943) The development of some external features in mouse embryos. J Hered 34:89–92
Hendrickx AG, Sawyer RH (1975) Embryology of the rhesus monkey. The Rhesus Monkey, Vol II, Academic Press Inc., New York
Hendrickx AG, Houston ML, Kraemer DC, Gasser RF, Bollert JA (1971) Embryology of the baboon. University of Chicago Press, Chicago
Hoshino K, Matsuzawa T, Murakami U (1973) Characteristics of the cell cycle of matrix cells in the mouse embryo during histogenesis of telencephalon. Expl Cell Res 77:89–94
Kauffman SL (1966) An autoradiographic study of the generation cycle in the ten-day mouse embryo neural tube. Expl Cell Res 42:67–73
Kauffman SL (1968) Lengthening of the generation cycle during embryonic differentiation of the mouse neural tube. Expl Cell Res 49:420–424
Kauffman SL (1969) Cell proliferation in embryonic mouse neural tube following urethane exposure. Dev Biol 20:146–157
LaVail JH, Cowan WM (1971) The development of the chick optic tectum. II. Autoradiographic studies. Brain Res28:421–441
O'Rahilly R, Gardner E (1971) The timing and sequence of events in the development of the human nervous system during the embryonic period proper. Z Anat Entwickl-Gesch, 134:1–12
Pierce ET (1973) Time of origin of neurons in, the brain stem of the mouse. Prog Brain Res 40:53–65
Rakic P (1974) Neurons in rhesus monkey visual cortex: systemic relation between time of origin and eventual disposition. Science 183:425–427
Sawyer RH, Wilson DB, Anderson J, Hendrickx AG (1974) Incorporation of 3H-thymidine in rhesus monkey (Macaca mulatta) embryos. J Exp Zool 189:121–126
Sidman RL (1967) Cell proliferation and migration in the developing brain. In: McKhann GM, Yafee SJ (eds) Drugs and poisons in relation to the developing nervous system. Proc Conf Drugs & Poisons, PHS Pub No 1791
Sidman RL (1970) Autoradiographic methods and principles for study of the nervous system with thymidine-H3. In: Nauta WJH, Ebbesson SOE (eds) Contemporary research methods in neuroanatomy. Springer, Berlin, pp 252–274
Streeter GL (1942) Developmental horizons in human embryos. Description of age group XI, 13 to 20 somites, and age group XII, 21 to 29 somites. Contr Embryol Carneg Inst 30:211–245
Streeter GL (1945) Developmental horizons in human embryos. Description of age group XIII, embryos about 4 or 5 millimeters long, and age group XIV, period of indentation of the lens vesicle. Contr Embryol Carneg Inst 31:27–63
Streeter GL (1948) Developmental horizons in human embryos. Description of age groups XV, XVI, XVII, and XVIII. Contr Embryol Carneg Inst 32:135–203
Streeter GL (1951) Developmental horizons in human embryos. Description of age groups XIX, XX, XXI, XXII, and XXIII, being the fifth issue of a survey of the Carnegie collection. Contr Embryol Carneg Inst 34:165–196
Theiler K (1972) The house mouse. Springer-Verlag, New York, pp 129–151
Theiler K (1979) Inbred and genetically defined strains of laboratory animals. In: Altman PL, Katz DD (eds) Part I. Mouse and rat. Fed Am Soc Exp Biol, Bethesda
Wilson DB (1973) Chronological changes in the cell cycle of chick neuroepithelial cells. J Embryol Exp Morphol 29:745–749
Wilson DB (1974a) The cell cycle of ventricular cells in the overgrown optic tectum. Brain Res 69:41–48
Wilson DB (1974b) Proliferation in the neural tube of the splotch (Sp) mutant mouse. J Comp Neurol 154:249–256
Wilson DB, Center EM (1974) The neural cell cycle, in the looptail (Lp) mutant mouse. J Embryol Exp Morphol 32:697–705
Wilson DB (1982) The cell cycle during closure of the neural folds in the C57BL mouse. Dev Brain Res (2:420–424)
Author information
Authors and Affiliations
Additional information
Supported by USPHS grants HD08658, RR00169, and HD09562
Rights and permissions
About this article
Cite this article
Wilson, D.B., Hendrickx, A.G. A comparative analysis of [3H]thymidine labeling in the embryonic tectum of the rhesus monkey (Macaca mulatta) and C57BL mouse. Anat Embryol 164, 277–285 (1982). https://doi.org/10.1007/BF00318511
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00318511