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Distribution of lamprey gonadotropin-releasing hormone-III (GnRH-III) in brains of larval lampreys (Petromyzon marinus)

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Abstract

Two immunoreactive forms of gonadotropinreleasing hormone (GnRH), lamprey GnRH-I and lamprey GnRH-III, were found in neurons in larval sea lampreys (Petromyzon marinus). Using antisera preferentially directed against either lamprey GnRH-I or-III, dense reaction product was seen in cell bodies in the rostral hypothalamus and preoptic area. Reaction product was also dense in fibers to and within the neurohypophysis, in addition to numerous fibers which projected caudally, beyond the neurohypophysis through the mesencephalon. The majority of immunoreactive GnRH was lamprey GnRH-III, and when lamprey GnRH-I was seen, it was in cells that appeared to contain both forms of GnRH. A small number of cells found in the caudal hypothalamus contained only immunoreactive lamprey GnRH-III, and these may constitute a functional subgroup within the population of GnRH neurons. In animals undergoing metamorphosis there was a large increase in reaction product in all GnRH-containing cells and fibers. A striking change within the distribution of GnRH cells was localized to a distinct group of GnRH-immunoreactive cells (GnRH-I and-III) in the ventral anterior hypothalamic area. These cells were minimally detectable in larvae, but during metamorphosis became densely filled with immunoreactive product in perikarya and distal processes. The results are consistent with the hypothesis that lamprey GnRH-III is an important form of GnRH during the maturation of GnRH cells and fibers, and further indicates that these cells have attained their normal positions in the preoptic area and hypothalamus before metamorphosis.

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Authors and Affiliations

  1. Laboratory of Developmental Neuroendocrinology, The Shriver Center, 02254, Waltham, MA, USA

    S. A. Tobet

  2. Program in Neuroscience, Harvard Medical School, 02115, Boston, MA, USA

    S. A. Tobet

  3. Primate Research Institute, Kyoto University, 484, Inuyama, Aichi, Japan

    M. Nozaki

  4. Department of Zoology, University of Toronto, M1C 1A4, Scarborough, Ontario, Canada

    J. H. Youson

  5. The Department of Biochemistry and Molecular Biology, University of New Hampshire, 03824, Durham, NH, USA

    S. A. Sower

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  1. S. A. Tobet
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  2. M. Nozaki
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  3. J. H. Youson
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  4. S. A. Sower
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Tobet, S.A., Nozaki, M., Youson, J.H. et al. Distribution of lamprey gonadotropin-releasing hormone-III (GnRH-III) in brains of larval lampreys (Petromyzon marinus). Cell Tissue Res 279, 261–270 (1995). https://doi.org/10.1007/BF00318482

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  • DOI: https://doi.org/10.1007/BF00318482

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