Summary
Mitochondrial DNA (mtDNA) has been isolated from four strains of Physarum polycephalum and a restriction site map has been determined using nine restriction enzymes. The restriction site maps of the four strains are similar but each strain is distinguished by insertions, deletions and restriction enzyme site polymorphisms. The sum of the restriction fragments gives mitochondrial genome sizes which vary from about 56 kb to 62 kb. In all four strains the composite map of the restriction enzyme sites for the mtDNA is circular. Knowledge of the restriction enzyme map has enabled cloning of mtDNA fragments representing the entire mtDNA of strain M3. The cloned fragments have been used to create a transcription map of the mtDNA.
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Alwine JC, Kemp DJ, Parker BA, Reiser J, Renart J, Stark GR, Wahl GM (1979) Methods Enzymol 68:220–242
Attardi G, Schatz G (1988) Annu Rev Cell Bio 4:289–333
Benne R, Sloof P (1987) BioSystems 21:51–68
Bohnert JH (1977) Exp Cell Res 106:426–430
Boyd DA, Hogman TC, Gruenke SA, Klassen GR (1984) Can J Biochem Cell Biol 62:571–576
Braun CJ, Levings CS III (1985) Plant Physiol 79:571–577
Campbell GR, Littau VC, Melera PW, Allfrey VG, Johnson EM (1979) Nucleic Acids Res 6:1433–1477
Daniel JW, Baldwin HH (1964) In: Prescott DM (ed) Methods in cell physiol, vol 1. Academic Press, New York pp 9–41
de Zamaroczy M, Bernardi G (1986) Gene 47:155–177
Dujon B (1983) In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983: nucleo-mitochondiral interactions. de Gruyter, Berlin, pp 1–24
Evans TE (1966) Biochem Biophys Res Comm 22:678–683
Evans TE, Suskind D (1971) Biochim Biophys Acta 228:350–364
Goddard JM, Cummings (1975) J Mol Biol 97:593–609
Goldbach RW, Bollen-de Boer JE, Van Bruggen EFJ, Borst P (1978) Biochim Biophys Acta 521:187–197
Grant D, Chiang KS (1980) Plasmid 4:82–96
Grossman LI, Hudspeth MES (1985) In: Bennett JW, Lasure LL (eds) Gene manipulations in fungi. Academic Press, New York, pp 65–103
Guttes E, Guttes S (1964) Science 145:1057–1058
Hasegawa M, Iida Y, Yano T, Takaiwa F, Iwabuchi M (1985) J Mol Evol 22:32–38
Hudspeth MES, Shumard DS, Bradford CJR, Grossman LI (1983) Proc Natl Acad Sci USA 80:142–146
Isaac PG, Brennicke A, Dunbar S, Leaver CJ (1985) Curr Genet 10:321–328
Johansen T, Johansen S, Haugli F (1988) Curr Genet 14:265–273
Kawano S, Susuki T, Kuroiwa T, (1982) Biochim Biophys Acta 696:290–298
Kawano S, Anderson RW, Nanba T, Kuroiwa (1987) J Gen Microbiol 133:3175–3182
Levings, CS, Brown GG (1989) Cell 56:171–179
McNabb SA, Boyd DA, Belkhiri A, Dick MW, Klassen GR (1987) Curr Genet 12:205–208
Margulis L, Schwartz KV (1988) The five kingdoms. Freeman Press, New York
Moritz C, Brown WM (1986) Science 233:1425–1427
Muhich M, Neckelmann N, Simpson L (1985) Nucleic Acids Res 13:3241–3260
Palmer JD (1985) Annu Rev Genet 19:325–354
Schuster W, Brennicke A (1986) Mol Gen Genet 204:29–35
Sederoff RR (1984) Adv Genet 22:1–108
Simpson L, Shaw H (1989) Cell 57:355–366
Sonenshein GE, Holt CE (1968) Biochem Biophys Res Comm 33:361–367
Southern EM (1975) J Mol Biol 89:503–517
Suyama Y, Miura K (1968) Proc Natl Acad Sci USA 60:235–242
Suyama Y, (1986) Curr Genet 10:411–420
Taylor JW (1986) Exp Mycol 10:463–467
Warrior R, Gall J (1986) Arch Sci (Geneva) 38:439–445
Wolf K, Del Giudice L (1988) Adv Genet 25:185–308
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Communicated by C. S. Levings III
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Jones, E.P., Mahendran, R., Spottswood, M.R. et al. Mitochondrial DNA of Physarum polycephalum: Physical mapping, cloning and transcription mapping. Curr Genet 17, 331–337 (1990). https://doi.org/10.1007/BF00314881
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DOI: https://doi.org/10.1007/BF00314881