Abstract
The peritoneal exudates of seabream and sea bass consist of granulocytes, lymphocytes and macrophages. These cells show conspicuous ultrastructural differences from the same cell-types of blood and head-kidney, which have not been reported previously. Peritoneal exudate granulocytes differ from their corresponding circulating or head-kidney forms in the following way: (a) they are larger in size, and (b) their abundant cytoplasmic granules have some new ultrastructural features, and a new granule population might also be present. Likewise, lymphocytes also show a noticeable difference; they contain a sparse population of small dense cytoplasmic granules. Monocytes, macrophages, and transitional forms between these two cell-types, are also found. The percentage of peritoneal exudate cell-types is different in seabream and sea bass. Macrophages in sea bass represent the most abundant peritoneal exudate cell-type. However, seabream shows lower percentages of macrophages than granulocytes.
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References
Absher M (1973) Hemocytometer counting. In: Kruse PK, Patterson MJ (eds) Tissue culture: methods and applications. Academic Press, New York
Bielek E (1980) Electron microscopical studies of blood cells in teleosts. IV. Monocytes and macrophages. Zool Jb Anat Bd 103:498–509
Bielek E (1981) Developmental stages and localization of peroxidatic activity in the leucocytes of three teleost species (Cyprinus carpio L.; Tinca tinca L.; Salmo gaidneri Richardson). Cell Tissue Res 220:163–180
Bielek E (1988) Ultrastructural analysis of leucocyte interaction with tumour targets in a teleost, Cyprinus carpio L. Dev Comp Immunol 12:809–821
Bodammer JE (1986) Ultrastructural observations on peritoneal exudate cells from the striped bass. Vet Immunol Immunopathol 12:127–140
Braun-Nesje R, Bertheussen K, Kaplan K, Seljelid R (1981) Salmonid macrophages: separation, “in vitro” culture and characterization. J Fish Dis 4:141–151
Braun-Nesje R, Kaplan G, Seljelid R (1982) Rainbow trout macrophages “in vitro”: morphology and phagocytic activity. Dev Comp Immunol 6:281–291
Brederoo P, Van der Meulen J, Mommaas-Kienhuis AM (1983) Development of the granule population in neutrophil granulocytes from human bone marrow. Cell Tissue Res 228:433–449
Brederoo P, Van der Meulen J, Daems WTh (1986) Ultrastructural localization of peroxidase activity in developing neutrophil granulocytes from human bone marrow. Histochemistry 84:445–453
Cannon MS, Mollenhauer HH, Eurell TE, Lewis DH, Cannon AM, Tompkins CH (1980) An ultrastructural study of the leukocytes of the channel catfish, Ictalurus punctatus. J Morphol 164:1–23
Cenini P (1984) The ultrastructure of leucocytes in carp (Cyprinus carpio). J Zool 204:509–520
Doggett TA, Harris JE (1989) Ultrastructure of the peripheral blood leucocytes of Oreochromis mossambicus. J Fish Biol 33:747–756
Ellis AE (1976) Leucocytes and related cells in the plaice Pleuronectes platessa. J Fish Biol 8:143–156
Ellis AE (1977) The leucocytes of fish: A review. J Fish Biol 11:453–491
Ellis AE (1982) Differences between the immune mechanisms of fish and higher vertebrates. In: Roberts RJ (ed) Microbial Diseases of Fish. Academic Press, London, pp 1–29
Ellis AE, Munroe ALS, Roberts RJ (1976) Defence mechanisms in fish. 1. A study of the phagocytic system and the fate of intraperitoneally injected particulate material in the plaice (Pleuronectes platessa L.). J Fish Biol 8:67–78
Ferguson HW (1976) The ultrastructure of plaice (Pleuronectes platessa) leucocytes. J Fish Biol 8:139–142
Finn JP, Nielson NO (1971) The inflammatory response of rainbow trout. J Fish Biol 3:463–478
Fletcher TC, White A (1987) Metabolic and immunological effects of endotoxin in the plaice, Pleuronectes platessa L J Fish Biol 31:81–85
Graham S, Jeffries AH, Secombes CJ (1988) A novel assay to detect macrophage bactericidal activity in fish. Factors influencing the killing of Aeromonas salmonicida. J Fish Dis 11:389–396
Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Moler A, Nielsen K, Nyengaard JR, Pakkenberg B, Sorensen FB, Vesterby A, West MJ (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Acta Pathol Microbiol Immunol Scand 96:379–394
Jakowska S (1956) Morphologie et nomenclature des cellules du sang des téléostéens. Rev Hematol 11:519–539
Lester RJG, Daniels BA (1976) The esosinophilic cell of the white sucker, Catostomus commersoni. J Fish Res Bd Can 33:139–144
López-Ruiz A, Esteban MA, Meseguer J (1992) Blood cells of the gilthead seabream (Sparus aurata L.). Light and electron microscopic studies. Anat Rec 234:161–171
MacArthur JI, Fletcher TC, Pirie BJS, Davidson RJL, Thomson AW (1984) Peritoneal inflammatory cells in plaice, Pleuronectes platessa L.: effects of stress and endotoxin. J Fish Biol 25:69–81
Meseguer J, Esteban MA, Garcia-Ayala A, López-Ruiz A, Agulleiro B (1990) Granulopoiesis in the head-kidney of the sea bass (Dicentrarchus labrax L.): an ultrastructural study. Arch Histol Cytol 53:287–296
Meseguer J, Esteban MA, Agulleiro B (1991) Stromal cells, macrophages and lymphoid cells in the head-kidney of sea bass (Dicentrarchus labrax L.). An ultrastructural study. Arch Histol Cytol 54:299–309
Olivier G, Moore AR, Fildes J (1992) Toxicity of Aeromonas salmonicida cells to Atlantic salmon Salmo salar peritoneal macrophages. Dev Comp Immunol 16:49–61
Page M, Rowley AF (1983) A cytochemical, light and electron microscopical study of the leucocytes of the adult river lamprey, Lampetra fluviatilis (L. Gray). J Fish Biol 22:503–517
Parish N, Wrathmell A, Harris JE (1985) Phagocytic cells in the dogfish (Scyliorhinus canicula L.). In: Manning MJ, Tatner MF (eds) Fish immunology. Academic Press, London, pp 71–83
Parish N, Wrathmell A, Hart S, Harris JE (1986) The leucocytes of the elasmobranch Scyliorhinus canicula L. A morphological study. J Fish Biol 28:545–561
Romestand B, Trilles JP (1984) Nomenclature and cytologie descriptive des éléments figurés du sang et des organes hémopoiétiques du bar (Dicentrarchus labrax). Rec Med Vet 160:833–840
Rowley AF, Hunt TC, Page M, Mainwaring G (1988) Vertebrate blood cells. In: Rowley AF, Ratcliffe NA (eds) Fish. Cambridge University Press, Cambridge, pp 19–127
Saggers BA, Gould ML (1989) The attachment of micro-organisms to macrophages isolated from the tilapia Oreochromis spilurus Gunther. J Fish Biol 35:287–294
Sakai DK (1984) Opsonization by fish antibody and complement in the immune phagocytosis by peritoneal exudate cells isolated from salmonid fishes. J Fish Dis 7:29–38
Sakai M, Ogasawara K, Atsuta S, Kobayashi M (1989) Comparative sensitivity of carp, Cyprinus carpio L. and rainbow trout, Salmo gairdneri Richardson, to Renibacterium salmoninarum. J Fish Dis 12:367–372
Savage AG (1983) The ultrastructure of the blood cells of the pike Esox lucius L. J Morphol 178:187–206
Suzuki K (1986) Morphological and phagocytic characteristics of peritoneal exudate cells in tilapia, Oreochromis niloticus (Trewavas), and carp, Cyprinus carpio L. J Fish Biol 29:349–364
Temkin RJ, McMillan DB (1986) Gut-associated lymphoid tissue (GALT) of the goldfish, Carassius auratus. J Morphol 190:9–26
Temmink JHM, Bayne CJ (1987) Ultrastructural characterization of leucocytes in the pronephros of carp (Cyprinus carpio L.). Dev Comp Immunol 11:125–137
Weeks BA, Keisler AS, Myrvik QN, Warinner JE (1987) Differential uptake of neutral red by macrophages from three species of estuarine fish. Dev Comp Immunol 11:117–124
Weinreb EL (1963) Studies on the fine structure of teleost blood cells. I. Peripheral blood. Anat Rec 147:219–238
Zuasti A, Ferrer C (1988) Granulopoiesis in the head kidney of Sparus auratus. Arch Histol Cytol 51:425–431
Zuasti A, Ferrer C (1989) Haemopoiesis in the head kidney of Sparus auratus. Arch Histol Cytol 52:249–255
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Meseguer, J., Esteban, M.A., Muñoz, J. et al. Ultrastructure of the peritoneal exudate cells of seawater teleosts, seabream (Sparus aurata) and sea bass (Dicentrarchus labrax). Cell Tissue Res 273, 301–307 (1993). https://doi.org/10.1007/BF00312832
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DOI: https://doi.org/10.1007/BF00312832