Abstract
Twenty-three fresh tumor specimens obtained at surgery and 5 serially transferable human tumor xenografts were implanted subcutaneously into nude mice and mice with severe combined immunodeficiency (SCID) to compare the take rates of the fresh surgical specimens and the growth rates of the transferable strains. The overall take rates were 65% for the SCID mice and 60% for the nude mice, without any significant difference, although colon carcinoma seemed to have higher acceptance in the SCID mice with a take rate of 6/8. All the serially transferable strains were successfully accepted in the SCID mice, their growth rates being essentially identical to those in the nude mice. These results indicate that the SCID mouse can be used as a human tumor xenograft-mouse system as well as the nude mouse.
References
Povlsen CO, Rygaard J (1971) Heterotransplantation of human adenocarcinomas of the colon and rectum to the mouse mutant nude. A study of nine consecutive transplantations. Acta Pathol Microbiol Scand [A] 79:159–169
Shimosato Y, Kameya T, Nagai K, Hirohashi S, Koide T, Hayashi H, Nomura T (1976) Transplantation of human tumors in nude mice. J Natl Cancer Inst 56:1251–1260
Bosma GC, Custer RP, Bosma MJ (1983) A severe combined immunodeficiency mutation in the mouse. Nature 301:527–530
Kubota T, Kubouchi K, Koh J, Enomoto K, Ishibiki K, Abe O (1983) Human breast carcinoma (MCF-7) serially transplanted into nude mice. Jpn J Surg 13:381–384
Geran RI, Greenberg NH, Schumacher AM, Abott BJ (1972) Protocols for screening chemical agents and natural products against animal tumors and other biological systems (3rd edn.). Cancer Chemother Rep 3:51–61
Kubota T, Nakada M, Tsuyuki K, Inada T, Asanuma F, Ishibiki K, Abe O (1986) Cell kinetics and chemosensitivity of human carcinomas serially transplanted into nude mice. Jpn J Cancer Res 77:502–507
Kubota T, Ishibiki K, Abe O (1988) The clinical usefulness of human xenografts in nude mice. In: Hall TC (ed) Prediction of response to cancer therapy. Alan R. Liss, New York, pp 213–225
Kubota T, Yamaguchi H, Watanabe M, Yamamoto T, Takahara T, Takeuchi T, Furukawa T, Kase S, Kodaira S, Ishibiki K, Kitajima M (1992) High human IgG levels in severe combined immunodeficient mouse reconstituted with human splenic tissues from patients with gastric cancer. Jpn J Cancer Res 83:300–303
Kubota T, Asanuma F, Kurihara H, Konno H, Koh J, Inada T, Kubochi K, Watanabe M, Suzuki K, Ishibiki K, Abe O (1984) The growth of human and murine tumors transplanted into nude mice with genetic backgrounds of BALB/cA, DDD and ICR (CD-1) (in Japanese with English abstract). Jpn J Cancer Chemother 11:104–110
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kubota, T., Yamaguchi, H., Watanabe, M. et al. Growth of human tumor xenografts in nude mice and mice with severe combined immunodeficiency (SCID). Surg Today 23, 375–377 (1993). https://doi.org/10.1007/BF00309059
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00309059