Summary
Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to study the innervation and vascular supply of crayfish skeletal muscle.
Blood vessels and nerve terminals identified by TEM were often closely associated. Synaptic regions of the nerve terminals were always located under sarcolemma and contained both dense-cored and agranular synaptic vesicles. Axo-axonal synapses of several different types were observed. Blood vessels consisted of several “vessel cells” or “supporting cells” enclosing a lumen, which was connected to the exterior by fine channels between the “supporting cells”.
SEM of whole freeze-dried muscles revealed two types of ramifying structure, which often ran in parallel over the muscle surface. One, identified as nerve, was more cylindrical and had a smoother surface than the other, which was identified as blood vessel. Fine nerve branches disappeared under the sarcolemma, probably near synaptic regions, but synapses could not be seen. Blood vessels also had fine terminations which merged into the sarcolemma.
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References
Abbott, J.: Absence of ood-brain barrier in a crustacean, Carcinus maenas L. Nature (Lond.) 225, 291–293 (1970).
Atwood, H.L.: Crustacean neuromuscular mechanisms. Amer. Zool. 7, 527–551 (1967).
Atwood, H.L., Hoyle, G., Smyth, T.: Mechanical and electrical responses of single innervated crab-muscle fibres. J. Physiol. (Lond.) 180, 449–482 (1965).
Atwood, H.L., Johnston, H.S.: Neuromuscular synapses of a crab motor axon. J. exp. Zool. 167, 457–470 (1968).
Atwood, H.L., Jones, A.: Presynaptic inhibition in crustacean muscle: axo-axonal synapse. Experientia (Basel) 23, 1036–1038 (1967).
Atwood, H.L., Morin, W. A.: Neuromuscular and axo-axonal synapses of the crayfish opener muscle. J. Ultrastruct. Res. 32, 351–369 (1970).
Bittner, G.D., Kennedy, D.: Quantitative aspects of transmitter release. J. Cell Biol. 47, 585–590 (1970).
Boyde, A., Wood, C.: Preparation of animal tissues for surface-scanning electron microscopy. J. Microsc, 90, 221–249 (1969).
Danini, E.S.: Beiträge zur vergleichenden Histologie des Blutes und des Bindegewebes. VI. Experimentell-histologische Untersuchungen über das Verhalten der Blutgefäßwand beim Flußkrebs (Potamobius leptodactylus). Jb. morph. mikr. Anat. 11, 565–597 (1927).
Harreveld, A. van: Doubly-, triply-, quadruply-, and quintuply-innervated crustacean muscles. J. comp. Neurol. 70, 285–296 (1939).
Harreveld, A. van, Wiersma, C.A.G.: The triple innervation of crayfish muscle and its function in contraction and inhibition. J. exp. Biol. 14, 448–461 (1937).
Hoy, R.R., Bittner, G.D., Kennedy, D.: Regeneration in crustacean motoneurons: evidence for axonal fusion. Science 156, 251–252 (1967).
Lockwood, A.P.M.: Aspects of the physiology of crustacea. San Francisco: W. H. Freeman and Co. 1967.
Maynard, D.M.: Circulation and heart function. In: The physiology of crustacea, vol. I (ed. T. H. Waterman), p. 161–226. New York: Academic Press (1960).
Nagy, I.Z.: Histochemical and electron microscope studies on the relation between dopamine and dense-core vesicles in the neurons of Anodonta cygnea L. Symposium on Neurobiology of Invertebrates (ed. J. Salanki), p. 69–84. New York: Plenum Press 1968.
Shivers, R.R.: Fine structure of crayfish optic ganglia. The University of Kansas Sci. Bull. 47, 677–733 (1967).
Shivers, R.R.: Fine structure of crayfish optic ganglia vascularization and permeability. J. Cell Biol. 47, 191A (1970).
Uchizono, K.: Inhibitory synapses on the stretch receptor neurone of the crayfish. Nature (Lond.) 214, 833–834 (1967).
Wiersma, C.A.G.: The neuromuscular system. In: The physiology of crustacea, vol. 2 (ed. T. H. Waterman), p. 191–240. New York: Academic Press 1961.
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Supported by grants from the National Research Council of Canada and The Muscular Dystrophy Association of Canada. The technical assistance of Mr. M. Uy is acknowledged. Dr. F. Lang held a Postdoctoral Fellowship from the Muscular Dystrophy Association of Canada. Acknowledgement is made for the use of the scanning electron microscope in the Royal Ontario Museum, established through a grant from N.R.C. to the Department of Zoology, University of Toronto for the development of a program in systematic and evolutionary Zoology.
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Lang, F., Atwood, H.L. & Morin, W.A. Innervation and vascular supply of the crayfish opener muscle: Scanning and transmission electron microscopy. Z.Zellforsch 127, 189–200 (1972). https://doi.org/10.1007/BF00306801
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DOI: https://doi.org/10.1007/BF00306801