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Electron microscopic demonstration of ornithine carbamoyltransferase in rat liver

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Summary

A method for electron microscopic demonstration of ornithine carbamoyltransferase (carbamyl phosphate: L ornithine carbamoyltransferase; E.C. 2.1.3.3) is described. The enzyme determination is based on precipitating the released orthophosphate with lead ions. Ornithine carbamoyl transferase activity is located in the mitochondrial matrix and in the cisternae of rough endoplasmic reticulum. The relation of citrulline synthesis in the urea cycle and ureidosuccinic acid synthesis competing for carbamoylphosphate is discussed.

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Authors and Affiliations

  1. II. Anatomisches Institut der Freien Universität Berlin, Germany

    H. -J. Merker

  2. Pharmakologisches Institut der Freien Universität Berlin, Germany

    S. Spors

Authors
  1. H. -J. Merker
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  2. S. Spors
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Additional information

The authors are grateful to Prof. Dr. D. Neubert for helpful discussions and criticism of the manuscript.

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Merker, H.J., Spors, S. Electron microscopic demonstration of ornithine carbamoyltransferase in rat liver. Histochemie 17, 83–88 (1969). https://doi.org/10.1007/BF00306334

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  • DOI: https://doi.org/10.1007/BF00306334

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