Summary
Yellow tetrazolium has been examined for use as a qualitative histochemical reagent in light microscopy. Experimental conditions for the demonstration of the lactate-yellow tetrazolium reductase system in mouse lung are described, and the possibility of subsequently demonstrating either NADH-MTT reductase or 3-glycerophosphate-MTT reductase in the same section has also been investigated. The yellow colour of the deposited formazan is uniquely favourable for counterstaining with blue nuclear stains.
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References
Barry, D.H., Robinson, W. E.: Enzyme histochemical studies on rat lungs. 2. Normal enzyme distribution in the alveolar tissue of the mature lung. Histochem. J. 1, 505–515 (1969).
Clara, M.: Zur Histobiologie des Bronchalepithels. Z. mikr.-anat. Forsch. 41, 321–347 (1937).
Etherton, J. E., Conning, D. M., Corrin, B.: Apocrine secretion of dipalmitoyl lecithin in the terminal bronchiole of mouse lung. I. Autoradiographical and morphological evidence (in preparation).
Fanchiotti, L. A. C. de, Jones, G. R. N., Bradbury, S.: The intramitochondrial localization of succinate-yellow tetrazolium reductase with the electron microscope. Histochemie 27, 28–35 (1971)
Farber, E., Sternberg, W. H., Dunlap, C. E.: Histochemical localization of specific oxidative enzymes. I. Tetrazolium stains for diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase. J. Histochem. Cytochem. 4, 254–265 (1956).
Jones, G. R. N.: The synthesis of yellow tetrazolium. Histochemie 18, 164–167 (1969a).
Jones, G. R. N.: Yellow tetrazolium: a new reagent to serve a new concept. Proc. roy. micr. Soc. 4, 79–80 (1969b).
Jones, G. R. N.: Coupled enzymic reactions involving tetrazolium salts. Proc. roy. micr. Soc. 5, 19 (1970).
Kalina, M., Gahan, P. B., Jones, G. R. N.: An evaluation of the histochemical demonstration of certain pyridine nucleotide—linked dehydrogenases. Nature (Lond.) 207, 647–648 (1965).
Pearse, A. G. E.: Histochemistry, theoretical and applied. London: J. and P. Churchill Ltd. (1961).
Scarpelli, D. G., Hess, R., Pearse, A. G. E.: The cytochemical localization of oxidative enzymes. I. Diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase. J. biophys. biochem. Cytol. 4, 747–752 (1958).
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This paper forms part of a thesis for the Ph. D. degree submitted to the Council for National Academic Awards by J.E.E.
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Etherton, J.E., Conning, D.M. & Jones, G.R.N. The demonstration of mouse lung lactate-yellow tetrazolium reductase. Histochemie 33, 287–290 (1973). https://doi.org/10.1007/BF00306257
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DOI: https://doi.org/10.1007/BF00306257