Summary
In man, apolipoprotein A-IV is characterized by a genetically determined polymorphism controlled by two codominant alleles. Two isoforms of this apolipoprotein, designated A-IV-1 and A-IV-2, can be identified by isoelectric focusing. Among 1000 healthy factory workers participating in an epidemiological study, A-IV-1 (genotype 1-1) was observed in 85%; A-IV-2 (genotype 2-2), in 0.5%; and A-IV-1 in combination with A-IV-2 (genotype 1–2), in 14%. In four nonrelated subjects, an apolipoprotein A-IV variant (A-IV-Münster), characterized by a slightly more basic isoelectric focusing behavior than A-IV-2, was detected in combination either with A-IV-1 or A-IV-2. Mendelian inheritance of this variant could be demonstrated.
Similar content being viewed by others
References
Assmann G, Oberwittler W, Schulte H, Schriewer H, Funke H, Epping PH, Hauss WH (1980) Prädiktion und Früherkennung der koronaren Herzkrankheit. Prospektive epidemiologische Studie bei Betriebsangehörigen im Raum Westfalen. Internist 21:446–459
Assmann G (1982) Lipoproteine und Atherosklerose. Schattauer, Stuttgart New York
Assmann G, Menzel HJ (1982) Apolipoprotein disorders. La Ricerca Clin Lab 12:63–81
Beisiegel U, Utermann G (1979) An apolipoprotein homolog of rat apolipoprotein A-IV in human plasma: isolation and partial characterization. Eur J Biochem 93:601–608
Green PHR, Glickman RM, Sandek CD, Blum CB, Tall AR (1979) Human intestinal lipoproteins. Studies in chyluric subjects. J Clin Invest 64:233–242
Green PHR, Glickman RM, Riley JW, Quinet E (1980) Human apolipoprotein A-IV. Intestinal origin and distribution in plasma. J Clin Invest 65:911–919
Imaizumi K, Fainaru M, Havel R (1978) Composition of proteins of mesenteric chylomicrons in the rat and alterations on exposure of chylomicrons to blood serum and serum proteins. J Lipid Res 19:712–722
Malik N, Berrie A (1972) New stain technique for proteins separated by gel isoelectric focusing based on Coomassie brilliant blue. Anal Biochem 49:173–176
Menzel HJ, Kladetzky RG, Assmann G (1982) One-step screening method for the polymorphism of apolipoprotein A-I, A-II and A-IV. J Lipid Res 23:915–922
Neville DM (1971) Molecular weight determination of protein-dodecylsulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem 246:6328–6334
Pagnan A, Havel RJ, Kane JP, Kotite L (1977) Characterization of human very low density lipoproteins containing two electrophoretic populations: double pre-beta lipoproteinemia and primary dysbetalipoproteinemia. J Lipid Res 18:613–622
Swaney BJ, Braithwaite V, Eder AH (1977) Characterization of apolipoproteins of rat plasma lipoproteins. Biochemistry 16:271–278
Utermann G, Feussner G, Franceschini G, Haas J, Steinmetz A (1982) Genetic variants of group A apolipoproteins. Rapid methods for screening and characterization without ultracentrifugation. J Biol Chem 257:501–507
Weisgraber KH, Bersot TP, Mahley RW (1978) Isolation and characterization of an apoprotein from the d<1.006 lipoproteins of human and canine lymph homologous with the rat A-IV apoprotein. Biochem Biophys Res Commun 85:287–292
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Menzel, HJ., Peter Kövary, M. & Assmann, G. Apolipoprotein A-IV polymorphism in man. Hum Genet 62, 349–352 (1982). https://doi.org/10.1007/BF00304554
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00304554