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Histochemical distribution of alkaline and acid phosphatase and adenosine triphosphatase in the brain of squirrel monkey (Saimiri sciureus)

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Summary

The distribution of some phosphatases (alkaline and acid phosphatase and ATPase) have been studied on 15 μ thick fresh frozen serial sections in the various regions and nuclei of the squirrel monkey brain. The alkaline phosphatase activity is concentrated in the blood vessels and the peripheral part of the neurons of some nuclei (e.g., nucleus supraoptic hypothalami) and the lining cells of choroid plexus. Acid phosphatase (AC) is a cellular enzyme and is concentrated in the large neurons of nuclei basalis Meynert, diagonalis band of Broca, magnocellular hypothalamic nuclei, corpus mammillaris, large sized neurons of the thalamus (e.g., nuclei paracentralis, ventralis lateralis, ventralis posterior thalami, magnocellular part of corpus geniculatum laterale), motor neurons of cranial nerve nuclei, large neurons of the reticular formation, giant pyramidal cells of cerebral cortex and the Purkinje cells of cerebellar cortex. The AC activity does not show as much variation in the different areas of the brain as the oxidative enzymes, which may mean that AC is more related to static maintenance metabolism of cells than to dynamic functional metabolism. The ATPase activity is more pronounced in the neuropil and the blood vessels compared to the neurons. In the perikarya, ATPase is concentrated close to the cell membrane, which may be significant in molecular transport across the membrane as well as in impulse conduction and synaptic transmission. Significant ATPase activity has been observed in the nucleus caudatus and putamen, magnocellular hypothalamic nuclei, nuclei parataenialis, paraventricularis, paracentralis, ventralis anterior thalami, habenular complex and cranial nerve nuclei.

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References

  • Adams, C. W. M.: Histochemistry of the cells in the nervous system. In: Neurohistochemistry (C. W. M. Adams, ed.), p. 253–331. Amsterdam: Elsevier Publ. Co. 1965.

    Google Scholar 

  • Albers, R. W.: Biochemical aspects of active transport. Ann. Rev. Biochem. 36, 727 (1967).

    Google Scholar 

  • —, Arnaiz, G. R., De Robertis, E.: Sodium potassium-activated p-nitrophenylphosphatase: a comparison of their subcellular localizations in rat brain. Proc. nat. Acad. Sci. (Wash.) 53, 557–564 (1965).

    Google Scholar 

  • Bannister, R. G., Romanul, R. C. A.: The localization of alkaline phosphatase activity in cerebral blood vessels. J. Neurol. Neurosurg. Psychiat. 26, 333 (1963).

    Google Scholar 

  • Barka, T., Anderson, P. J.: Histochemistry: theory, practice, and bibliography. New York: Hoeber Medical Division, Harper and Row, Publishers 1963.

    Google Scholar 

  • Becker, N. H., Goldfischer, S., Shin, Woo-Yung, Novikoff, A. B.: The localization of enzyme activities in the rat brain. J. biophys. biochem. Cytol. 8, 649–663 (1960).

    Google Scholar 

  • Bonting, S. L., Simon, K. A., Hawkins, N. M.: Studies on sodium-potassium activated adenosine triphosphatase. I. Quantitative distribution in several tissues of the cat. Arch. Biochem. 95, 416–423 (1961).

    Google Scholar 

  • Brun, A., Brunk, U.: Histochemical study of heavy metals in the rat brain at various ages. Acta path. microbiol. scand. 72, 451–452 (1968).

    Google Scholar 

  • Emmers, R., Akert, K.: A stereotaxic atlas of the brain of squirrel monkey. Madison: The University of Wisconsin Press 1963.

    Google Scholar 

  • Essner, E., Novikoff, A. B.: Localization of acid phosphatase activity in hepatic lysosomes by means of electron microscopy. J. biophys. biochem. Cytol. 9, 773–784 (1961).

    Google Scholar 

  • Fahn, S., Côté, L. J.: Regional distribution of sodium-potassium activated adenosine triphosphatase in the brain of the rhesus monkey. J. Neurochem. 15, 433 (1968).

    Google Scholar 

  • Friede, R. L., Knoller, M.: A quantitative mapping of acid phosphatase in the brain of the rhesus monkey. J. Neurochem. 12, 441 (1965).

    Google Scholar 

  • Gomori, G.: Microtechnical demonstration of phosphatase in tissue sections. Proc. Soc. exp. Biol. (N. Y.) 42, 23–26 (1939).

    Google Scholar 

  • —: An improved histochemical technique for acid phosphatase. Stain Technol. 25, 81–85 (1950).

    Google Scholar 

  • —: Alkaline phosphatase of cell nuclei. J. Lab. clin. Med. 37, 526–531 (1951).

    Google Scholar 

  • —: Microscopic histochemistry: principles and practice. Chicago, Illinois: Univ. of Chicago Press 1952.

    Google Scholar 

  • Grogg, E., Pearse, A. G. E.: A critical study of the histochemical techniques for acid phosphatase. J. Path. Bact. 64, 627 (1952).

    Google Scholar 

  • Hosie, R. J. A.: The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea pig brain. Biochem. J. 96, 404–412 (1965).

    Google Scholar 

  • Iijima, K., Shantha, T. R., Bourne, G. H.: Histochemical studies on the nucleus basalis of Meynert of the squirrel monkey (Saimiri sciureus). Acta histochem. (Jena) 30, 96–108 (1968).

    Google Scholar 

  • Jongkind, J. F., Swaab, D. F.: The distribution of thiamine diphosphate-phosphohydrolase in the neurosecretory nuclei of the rat following osmotic stress. Histochemie 11, 319–324 (1967).

    Google Scholar 

  • Josephy, H.: Acid phosphatase in the senile brain. Arch. Neurol. Psychiat. (Chic.) 6, 164–169 (1949).

    Google Scholar 

  • Kumamoto, T., Bourne, G. H.: Experimental studies on the oxidative enzymes and hydrolytic enzymes in spinal neurons. I. A histochemical and biochemical investigation of the spinal ganglion and the spinal cord following sciatic neurotomy in the guinea pig. Acta anat. (Basel) 55, 255–277 (1963).

    Google Scholar 

  • Kurokawa, M., Sakamoto, T., Kato, M.: Distribution of sodium plus potassium stimulated adenosine triphosphatase activity in isolated nerve ending particles. Biochem. J. 97, 833–844 (1965).

    Google Scholar 

  • Landers, J. W., Chason, J. L., Gonzalez, J. E., Palutke, W.: Morphology and enzymatic activity of rat and cerebral capillaries. Lab. Invest. 11, 1253–1259 (1962).

    Google Scholar 

  • Manocha, S. L., Bourne, G. H.: Histochemical mapping of monoamine oxidase and lactic dehydrogenase in the pons and mesencephalon of squirrel monkey (Saimiri sciureus). J. Neurochem. 13, 1047–1056 (1966 a).

    Google Scholar 

  • —: Histochemical mapping of succinic dehydrogenase and cytochrome oxidase in the spinal cord, medulla oblongata and cerebellum of squirrel monkey (Saimiri sciureus). Exp. Brain Res. 2, 216–229 (1966 b).

    Google Scholar 

  • —: Histochemical mapping of succinic dehydrogenase and cytochrome oxidase in the pons and mesencephalon of squirrel monkey (Saimiri sciureus). Exp. Brain Res. 2, 230–246 (1966 c).

    Google Scholar 

  • —: Histochemical mapping of succinic dehydrogenase and cytochrome oxidase in the diencephalon and basal telencephalic centers of the brain of squirrel monkey (Saimiri stciureus). Histochemie 9, 300–319 (1967).

    Google Scholar 

  • —: Histochemical mapping of lactate dehydrogenase and monoamine oxidase in the medulla oblongata and cerebellum of squirrel monkey (Saimiri sciureus). J. Neurochem. 15, 1033–1040 (1968).

    Google Scholar 

  • —, Shantha, T. R.: Enzyme histochemistry of the nervous system of rhesus monkey (Macaca mtulatta). New York: Academic Press 1970.

    Google Scholar 

  • —, Bourne, G. H.: Histochemical mapping of the distribution of monoamine oxidase in the diencephalon and basal telencephalic centers of the brain of squirrel monkey (Saimiri sciureus). Brain Res. 6, 570–586 (1967 a).

    Google Scholar 

  • —: Histochemical studies on the spinal cord of squirrel monkey (Saimiri sciureus). Exp. Brain Res. 3, 25–39 (1967 b).

    Google Scholar 

  • —, Bourne, G. H.: A stereotaxic atlas of the brain of the cebus monkey (Cebus apella). Oxford: Oxford University Press 1968.

    Google Scholar 

  • Meyer, P.: Histochemistry of the developing human brain. I. Alkaline phosphatase, acid phosphatase and AS-esterase in the cerebellum. Acta neurol. scand. 39, 123–138 (1963).

    Google Scholar 

  • Novikoff, A. B.: Lysosomes in the physiology and pathology of cells: contributions of staining methods. In: Ciba Foundation symp., lysosomes (A. V. S. de Reuck and M. P. Cameron, eds.), p. 36–77. Boston: Little Brown and Company 1963.

    Google Scholar 

  • Osinchak, J.: Electron microscopic localization of acid phosphatase and thiamine pyro- phosphatase activity in hypothalamic neurosecretory cells of the rat. J. Cell Biol. 21, 35–49 (1964).

    Google Scholar 

  • Padykula, H. A., Herman, E.: Factors affecting the activity of adenosine triphosphatase and other phosphatases as measured by histochemical techniques. J. Histochem. Cytochem. 3, 161–169 (1955 a).

    Google Scholar 

  • —: The specificity of the histochemical method for adenosine triphosphatase. J. Histochem. Cytochem. 3, 170–195 (1955 b).

    Google Scholar 

  • Pilgrim, C.: Enzyme histochemistry studies on thirst-activated neurosecretory cells in rats. Experientia (Basel) 23, 943 (1967).

    Google Scholar 

  • Samorajski, T., McCloud, J.: Alkaline phosphomonoesterase and blood-brain permeability. Lab. Invest. 10, 492–501 (1961).

    Google Scholar 

  • Sandler, M., Bourne, G. H.: Intranuclear histochemical localization of adenosine triphosphatase. J. Histochem. Cytochem. 10, 636 (1962).

    Google Scholar 

  • Schiffer, D., Fabiani, A., Monticone, G. F.: Acid phosphatase and non-specific esterase in normal and reactive glia of human nervous tissue. A histochemical study. Acta neuropath. (Berl.) 9, 316–327 (1967).

    Google Scholar 

  • Shantha, T. R., Manocha, S. L., Bourne, G. H.: Enzyme histochemistry of the mesenteric and dorsal root ganglion cells of cat and squirrel monkey. Histochemie 10, 234–245 (1967).

    Google Scholar 

  • —: Enzyme histochemistry of the cerebral cortex of squirrel monkey and rat. Acta histochem. (Jena) 30, 218–233 (1968 a).

    Google Scholar 

  • —: A stereotaxic atlas of the Java monkey brain (Macaca irus). Basel: Karger 1968 b.

    Google Scholar 

  • Shimizu, N.: Histochemical studies on the phosphatases of the nervous system. J. comp. Neurol. 93, 201–218 (1950).

    Google Scholar 

  • Skou, J. C.: Enzymatic basis for active transport of Na+ and K+ across cell membranes. Physiol. Rev. 45, 596–617 (1965).

    Google Scholar 

  • Sumner, J. B., Somers, G. F.: Chemistry and methods of enzymes. New York: Academic Press 1943.

    Google Scholar 

  • Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatases at a physiologic pH. Amer. J. clin. Path. 27, 13–23 (1957).

    Google Scholar 

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Authors and Affiliations

  1. Yerkes Regional Primate Research Center, Emory University, Atlanta, Georgia, USA

    Sohan L. Manocha

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  1. Sohan L. Manocha
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This work has been carried out with the aid of Grant No. 00165 from The Animal Resources Branch, National Institute of Health and a grant (NGR-11-001-016) from The National Aeronautics and Space Administration. Thanks are due to Mrs. M. J. Nimnicht and Miss M. E. Rogero for their technical help.

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Manocha, S.L. Histochemical distribution of alkaline and acid phosphatase and adenosine triphosphatase in the brain of squirrel monkey (Saimiri sciureus). Histochemie 21, 221–235 (1970). https://doi.org/10.1007/BF00304214

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