Summary
Cytochrome oxidase, succinic dehydrogenase, phosphorylase and branching enzymes were consistently demonstrated in frozen dried paraffin embedded dog myocardium if the appropriate solvent for deparaffinization was used. No modification of the incubating medium was necessary for cytochrome oxidase. For succinic dehydrogenase a 0.8 M succinate solution was used instead of the usual 0.2 M solution and phenazine methosulfate had to be added to the incubating medium. No primer or activators were necessary for demonstration of phosphorylase and branching enzyme activities.
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This work was supported by grant number HE-07605-06 from the National Heart Institute, National Institutes of Health, Bethesda, Md.
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Eckner, F.A.O., Riebe, B.H., Moulder, P.V. et al. Histochemical study of enzyme systems in frozen dried tissue. Histochemie 13, 283–288 (1968). https://doi.org/10.1007/BF00303762
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DOI: https://doi.org/10.1007/BF00303762