Summary
Atrial natriuretic peptide (ANP) levels in cardiocytes and plasma were examined by using immunohistochemistry, electron microscopy, and radioimmunoassay in non-obese diabetic mice (NOD). Cardiocyte ANP mRNA expression was measured by the polymerase chain reaction method. ANP immunoreactivity in the auricular cardiocytes was more prominent in hyperglycemic mice (NOD-h) than in normoglycemic mice (NOD-n). Ultrastructural examination showed that auricular cardiocytes of the NOD-h group contained more cytoplasmic granules than cells of the NOD-n group. Ultrastructural morphometry indicated that the number of granules per auricular cardiocyte was significantly larger in the NOD-h group than in the NOD-n group. (P<0.01), whereas the granule diameter was significantly smaller in the NOD-h group (P<0.01). Radioimmunoassay showed that ANP levels in the NOD-h auricular cardiocytes were significantly higher than those in the NOD-n cardiocytes (P<0.01); the opposite was true in plasma. Cardiocyte ANP mRNA expression was lower in the NOD-h group than in the NOD-n group.
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Bold de AJ (1979) Heart atria granularity effects of changes in water-electrolyte balance. Proc Soc Exp Biol Med 161:508–511
Bold de AJ (1982) Tissue fractionation studies on the relationship between an atrial natriuretic factor and specific atrial granules. Can J Physiol Pharmacol 60:324–330
Cantin M, Gutkowska J, Thibault G, Milne RW, Ledoux S, Chapeau C, Garcia P, Hamet P, Genest J (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Histochemistry 80:113–127
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
Christlieb AR, Kaldany A, D'Elia JA (1976) Plasma renin activity and hypertension in diabetes mellitus. Diabetes 25:969–974
Feldt-Rasmussen B, Mathiesen ER, Deckert T, Giese J, Christensen NJ, Bent-Hansen L, Nielsen MD (1987) Centrla role for sodium in the pathogenesis of blood pressure changes independent of angiotensin, aldosterone and catecholamines in type I (insulin-dependent) diabetes mellitus. Diabetologia 30:610–617
Fujita T, Yui R, Kusumoto Y, Serizawa Y, Makino S, Tochino Y (1982) Lymphocytic insulitis in a ‘non-obese diabetic (NOD)’ strain of mice: an immunohistochemical and electron microscope investigation. Biomed Res 3:429–443
Fushimi H, Nonaka K, Tarui S, Tochino Y, Kanaya H (1980) The effects of parabiosis on serum and kidney glycosidase activities in spontaneously diabetic mice. Diabetologia 19:50–53
Gall JAM, Alcorn D, Fernley R, Coghlan JP, Ryan GB (1990) Qualitative and quantitative analysis of granules in atrial appendage cardiocytes in different physiological states. Cell Tissue Res 259:529–534
Jungmann E, Walter-Schrader MC, Haak T, Fassbinder W, Wambach G, Althoff PH, Schoffling K (1988) Impaired renal responsiveness to human atrial natriuretic peptide (hANP) in normotensive patients with type I diabetes mellitus. Klin Wochenschr 66:527–532
Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-hANP). Biochem Biophys Res Commun 118:131–139
Kangawa K, Fukuda A, Matsuo H (1985) Structural identification of β- and γ-human atrial natriuretic polypeptides. Nature 313:397–400
Kimura T, Abe K, Ota K, Omata K, Shoji M, Kubo K, Matsui K, Inoue M, Yasujima M, Yoshinaga K (1986) Effects of acute water load, hypertonic saline infusion, and furosemide administration on atrial natriuretic peptide and vasopressin release in humans. J Clin Endocrinol Metab 62:1003–1010
Makino S, Kunimoto K, Muraoka Y, Katagiri K (1980) Breeding of a non-obese, diabetic strain of mice. Exp Anim 29:1–13
Mifune H, Suzuki S, Noda Y, Mohri S, Mochizuki K (1991a) Fine structure of atrial natriuretic peptide (ANP)-granules in the atrial cardiocytes of the mouse, rat and Mongolian gerbil. Exp Anim 40:183–193
Mifune H, Suzuki S, Noda Y, Mohri S, Mochizuki K (1991b) Fine structure of atrial natriuretic peptide (ANP)-granules in the cardiocytes in the pig, cattle and horse. J Vet Med Sci 53:561–568
Nakao K, Sugawara A, Morii N, Sakamoto M, Suda M, Soneda J, Ban T, Kihara M, Yamori Y, Shimokura M, Kiso Y, Imura H (1984) Radioimmunoassay for α-human and rat atrial natriuretic polypeptide. Biochem Biophys Res Commun 124:815–821
Nakayama K, Ohkubo H, Hirose T, Inayama S, Nakanishi S (1984) mRNA sequence for human cardiodilatin-atrial natriuretic factor precursor and regulation of precursor mRNA in rat atria. Nature 23:699–701
O'Hare JP, Roland JM, Walters G, Corrall RJM (1986) Impaired sodium excretion in response to volume expansion induced by water immersion in insulin-dependent diabetes mellitus. Clin Sci 71:403–409
Ohneda A, Kobayashi T, Nihei J, Tochino Y, Kanaya H, Makino S (1984) Insulin and glucagon in spontaneously diabetic nonobese mice. Diabetologia 27:460–463
Sagnella GA, Markandu ND, Shore AC, MacGregor GA (1987) Plasma immunoreactive atrial natriuretic peptide and changes in dietary sodium intake in man. Life Sci 40:139–143
Schwartz D, Katsube NC, Needleman P (1986) Atriopeptin release in conditions of altered salt and water balance in the rat. Biochem Biophys Res Commun 137:922–928
Seidman CE, Block KD, Klein KA, Smith JA, Seidman JG (1984) Nucleotide sequences of the human and mouse atrial natriuretic factor gene. Science 226:1206–1209
Stein BA, Katzeff I, Norton G, DeWet G, Rosendorff C (1990) Differental size distribution of atrial dense granules in spontaneously hypertensive, Wistar-Kyoto and Wistar rats. Acta Anat 137:331–335
Takayanagi R, Tanaka I, Maki M, Inagami T (1985) Effects of changes in water-sodium balance on levels of atrial natriuretic factor messenger RNA and peptide in rats. Life Sci 36:1843–1848
Thibault G, Garcia R, Cantin M, Genest J (1983) Atrial natriuretic factor. Characterization and partial purification. Hypertension 5 [Suppl I]:75–80
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Mifune, H., Suzuki, S., Honda, J. et al. Atrial natriuretic peptide (ANP): a study of ANP and its mRNA in cardiocytes, and of plasma ANP levels in non-obese diabetic mice. Cell Tissue Res 267, 267–272 (1992). https://doi.org/10.1007/BF00302964
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DOI: https://doi.org/10.1007/BF00302964