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Morphological and histochemical differentiation of intrafusal fibres in the Posterior Latissimus dorsi muscle of the developing chick

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Summary

Morphological and histochemical differentiation of neuromuscular spindles was studied in the posterior latissimus dorsi (PLD) of the chick during embryonic and post-hatching development. A rapid increase in the number of spindles takes place between the 13th and 15th of embryonic life. By the 15th day in ovo, the spindle capsule appears filled with numerous contiguous cells. Large sensory endings and small primitive motor endings are observed on intrafusal fibres. Ultrastructural observations of the nerve supply of the spindles confirm that each developing spindle receives one thick Ia axon with one to three thin γ axons. The intracapsular space differentiates by the 17th day of embryonic development. All intrafusal fibres are morphologically of the nuclear-chain type, while two fibre types are distinguished as early as the 14th day of embryonic life, when myofibrillar ATPase activity is demonstrated after acid preincubation. These two histochemical types of intrafusal fibres are also described in the adult. The relation between these two histochemical types and different functional activity of intrafusal fibres is suggested.

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Authors and Affiliations

  1. Laboratoire de Physiologie animale et cellulaire Faculté des Sciences, 2 rue de la Houssinière, F-44072, Nantes Cédex, France

    M. Toutant, T. Rouaud & J. P. Toutant

  2. Départment de Biologie Moléculaire Institut Pasteur, 25 rue du Docteur Roux, F-75724, Paris Cédex 15, France

    J. P. Bourgeois

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  1. M. Toutant
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  2. J. P. Bourgeois
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  3. T. Rouaud
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  4. J. P. Toutant
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Toutant, M., Bourgeois, J.P., Rouaud, T. et al. Morphological and histochemical differentiation of intrafusal fibres in the Posterior Latissimus dorsi muscle of the developing chick. Anat Embryol 162, 325–342 (1981). https://doi.org/10.1007/BF00299976

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