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Inhibition of methionine uptake by methotrexate in mouse leukemia L1210 cells

  • Original Articles
  • Methionine, Methotrexate, L1210 Cells
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Summary

Methionine-auxotrophic L1210 cells were used to study the effect of methotrexate (MTX) on methionine utake and metabolism. MTX was shown to inhibit amino acid transport systems and cause a decrease of methionine uptake into L1210 cells. Conversely, a nonmetabolizable amino acid analogue reduced MTX uptake into L1210 cells. MTX also blocked the transfer of the beta carbon from serine into methionine. Therefore, methionine deprivation may be an additional mechanism of action for MTX in methionine-auxotrophic tumor cells.

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Authors and Affiliations

  1. Section of Biochemical Pharmacology, Department of Medical Oncology, City of Hope National Medical Center, 91010, Duarte, CA, USA

    Kevin J. Scanlon, Mohammed Kashani-Sabet, Michele Pallai & Maria Saketos

  2. Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, CT, USA

    Arlene R. Cashmore, Robert N. Dreyer & Barbara A. Moroson

Authors
  1. Kevin J. Scanlon
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  2. Arlene R. Cashmore
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  3. Mohammed Kashani-Sabet
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  4. Michele Pallai
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  5. Robert N. Dreyer
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  6. Barbara A. Moroson
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  7. Maria Saketos
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Additional information

This work was supported by NIH grants USPHS CA 08010 and CA 16359, the American Cancer Society (CH-265), and the Chemotherapy Foundation, Inc.

Kevin J. Scanlon is a Scholar of the Leukemia Society of America

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Scanlon, K.J., Cashmore, A.R., Kashani-Sabet, M. et al. Inhibition of methionine uptake by methotrexate in mouse leukemia L1210 cells. Cancer Chemother. Pharmacol. 19, 21–24 (1987). https://doi.org/10.1007/BF00296249

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  • DOI: https://doi.org/10.1007/BF00296249

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