Summary
Formalin-fixed and paraffin-embedded specimens of 24 human gliomas were examined histochemically with five lectins: concanavalin A (Con A), wheat germ agglutinin (WGA), Ricinus communis agglutinin 1 (RCA-1), peanut agglutinin (PNA), and Ulex europaeus agglutinin 1 (UEA-1). Although the staining intensity with lectins was variable, tumor cells in five astrocytomas, three oligodendrogliomas, six ependymomas, and one gliosarcoma, were generally positive for Con A, WGA, and RCA-1, and negative for PNA and UEA-1, whereas those in nine glioblastomas were usually positive for Con A and WGA and negative for RCA-1 and PNA as well as UEA-1. The malignancy in neoplastic astrocytes was correlated with the decrease in binding with lectins, especially RCA-1. Blood vessels, particularly the endothelial layers, in all gliomas were stained intensely with all lectins used. Macrophages showed two staining features with lectins; stippled and granular. The former macroplìages were positive for Con A, WGA, RCA-1, and PNA, and negative for UEA-1, whereas the latter macrophages were positive for all lectins used. Thus, the staining characteristics with lectins of macrophages were different from those of any glioma cells and very useful for identification of macrophages in gliomas.
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References
Alroy J, Adelman LS, Warren CD (1988) Lectin histochemistry of gangliosidosis. II. Neurovisceral tissues from patients with Sandhoff's disease. Acta Neuropathol 76:359–365
Böhling T, Paetau A, Ekblom P, Haltia M (1983) Distribution of endothelial and basement membrane markers in angiogenic tumors of the nervous system. Acta Neuropathol (Berl) 62:67–72
Damjanov I (1987) Biology of disease. Lectin cytochemistry and histochemistry. Lab Invest 57:5–20
Damjanov I, Black P (1987) Lectin binding sites on the luminal surface of ependymal cells of the rat spinal cord: implications for neuropathological investigation. Neurosurgery 20:722–725
Feizi T (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are oncodevelopmental antigens. Nature 314:53–57
Goldstein IJ, Poretz RD (1986) Isolation physicochemical characterization, and carbohydrate-binding specificity of lectins. In: Liemer FE, Sharon N, Goldstein IJ (eds) The lectins: properties, functions and applications in biology and medicine. Academic Press, Orlando pp 35–247
Hakomori S-I (1985) Aberrant glycosylation in cancer cell membranes as focused on glycolipids: overview and perspectives. Cancer Res 45:2405–2414
Hampson DR, Poduslo SE (1987) Comparisons of proteins and glycoproteins in neuronal plasma membranes, axolemma, synaptic membranes, and oligodendroglial plasma membranes. J Neurosci Res 17:277–284
Hitchcock ER, Morris CS (1988) Mononuclear cell infiltration in central portions of human astrocytomas. J Neurosurg 68:432–437
Howard DR, Batsakis JG (1982) Peanut agglutinin: a new marker for tissue histiocytes. Am J Clin Pathol 77:401–408
Hsu S-M, Jaffe ES (1984) Leu M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's disease. Am J Clin Pathol 82:29–32
Hsu S-M, Raine L (1982) Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections. J Histochem Cytochem 30:157–161
Hsu S-M, Ree HJ (1983) Histochemical studies on lectin binding in reactive lymphoid tissues. J Histochem Cytochem 31:538–546
Kobori N, Ibayashi N, Yoshino E, Nakagawa Y, Suzuki K, Ueda S, Hirakawa K (1988) Lectin-binding pattern in ependymoma and oligodendroglioma. Brain Tumor Pathol 5:25–32 (in Japanese)
Kochi N, Budka M, Radaszkiewicz T (1986) Development of stroma in malignant lymphomas of the brain compared with epidural lymphomas. Acta Neuropathol (Berl) 71:125–129
Mannoji H, Yeger H, Becker LE (1986) A specific histochemical marker (lectin Ricinus communis agglutinin-1) for normal human microglia, and application to routine histopathology. Acta Neuropathol (Berl) 71:341–343
Miettinen M, Holthofer H, Lehto V-P, Miettinen A, Virtanen I (1983) Ulex europaeus I lectin as a marker for tumors derived from endothelial cells. Am J Clin Pathol 79:32–36
Möller P (1982) Peanut lectin: a useful tool for detecting Hodgkin cells in paraffin sections. Virchows Arch [A] 396:313–317
Ordonez NG, Batsakis JG (1984) Comparison of Ulex europaeus I lectin and factor VIII-related antigen in vascular lesions. Arch Pathol Lab Med 108:129–132
Palma L, Lorenzo ND, Guidetti B (1978) Lymphocytic infiltrates in primary glioblastomas and recidivous gliomas. J Neurosurg 49:854–861
Phillips JP, Eremin O, Anderson JR (1982) Lymphoreticular cells in human brain tumors and in normal brain. Br J Cancer 45:61–69
Ree HJ (1986) Concanavalin A-binding histiocytes in Hodgkin's disease. Cancer 58:87–95
Ree HJ, Kadin ME (1985) Macrophages-histiocytes in Hodgkin's disease: the relation of peanut agglutinin (PNA)-binding macrophage-histiocytes to clinicopathologic presentation and course of disease. Cancer 56:333–338
Ree HJ, Kadin ME (1985) Lectin distinction of benign from malignant histiocytes. Cancer 56:2046–2050
Ree HJ, Kadin ME (1986) Peanut agglutinin: a useful marker for histiocytosis X and interdigitating reticulum cells. Cancer 57:282–287
Roholl PJM, Kleyne J, Pijpers HW, Van Unnik JAM (1985) Comparative immunohistochemical investigation of markers for malignant histiocytes. Hum Pathol 16:763–771
Rossi ML, Hughes JT, Esiri MM, Coakham HB, Brownell DB (1987) Immunohistological study of mononuclear cell infiltrate in malignant gliomas. Acta Neuropathol (Berl) 74:269–277
Schwechheimer K, Schnabel P, Möller P (1983) Immunohistochemical localization of peanut lectin binding sites on human brain tumors as determined by peroxidase-antiperoxidase technique in paraffin sections. Acta Neuropathol (Berl) 61:21–26
Schwechheimer K, Weiss G, Schnabel P, Möller P (1984) Lectin target cells in human central nervous system and the pituitary gland. Histochemistry 80:165–169
Stanojev D, Gurd JW (1987) Characterization of fucosyl oligosaccharides associated with synaptic membrane and synaptic junctional glycoproteins. J Neurochem 48:1604–1611
Szumanska G, Virbrodt AW, Mandybur TI, Wisniewski HM (1987) Lectin histochemistry of plaques and tangles in Alzheimer's disease. Acta Neuropathol (Berl) 73:1–11
Weber T, Seitz RJ, Liebert UG, Gallasch E, Wechsler W (1985) Affinity cytochemistry of vascular endothelia in brain tumors by biotinylated Ulex europaeus type I lectin (UEA-I). Acta Neuropathol (Berl) 67:128–135
Wood GW, Morantz RA (1979) Immunohistologic evaluation of the lymphoreticular infiltrate of human central nervous system tumors. J Natl Cancer Inst 62:485–491
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Wang, X.C., Kochi, N., Tani, E. et al. Lectin histochemistry of human gliomas. Acta Neuropathol 79, 176–182 (1989). https://doi.org/10.1007/BF00294376
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DOI: https://doi.org/10.1007/BF00294376