Summary
Certain important aspects of the urine oxalate tolerance test (OTT) have been revised. The stirring system has been changed and the test has been adapted to the kinetics of calcium oxalate precipitation. True equilibrium conditions are now ensured during the measurements. Furthermore, the endogenous oxalate concentration is determined and taken into consideration. As a result of these changes, the significance of the test results has greatly improved. The effects of the addition of small amounts of zinc on the precipitation of calcium oxalate have been used in a new variation of the OTT. This new test makes it possible to discriminate much faster and more simply between recurrent stone-formers and other subjects. Tamm-Horsfall protein (THP) has been tested for its effect on the precipitation of calcium oxalate by means of OTT. THP inhibits the precipitation of calcium oxalate, but THP of stone-formers has a diminished inhibitory activity. The inhibitory activity of this protein strongly depends on the method by which it is isolated.
Similar content being viewed by others
References
Achilles W (1985) The gel crystallization method. Fortschr Urol Nephrol 23:252
Baumann JM, Lauber K, Lustenberger FX, Wacker M, Zingg EJ (1985) The direct measurement of inhibitor capacity in urine. Urol Res 13:169
Berg W, Schneider HJ, Brundig P, Bother C (1981) Verrechnungsmöglichkeiten und Patienten mit Kalziumoxalatlithiasis. Fortschr Urol Nephrol 20:21
Bichler KH, Kirchner CH, Ideler V (1976) Uromucoid excretion of normal individual and stone formers. Br J Urol 47:733
Briellmann T, Seiler H, Hering F, Rutishauser G (1985) The oxalate tolerance method. Urol Res 13:291
Finlayson B, Reid F (1978) Invest Urol 15:442
Hallson PC, Rose GA (1978) Rapid evaporation technique. Br J Urol 50:442
Hering F, Pyhel N, Ratajcak H, Friedrich R, Lutzeyer W (1981) Kalkulation der relativen Urinsättingung-Entscheidungshilfe bei der Therapieplanung. Fortsch Urol Nephrol 17:345
Ljunghall S, Danielson BG, Källsen R, Fritjofson A. Prediction of stone recurrence. In: Smith LH, Robertson WG, Finlayson B (eds) Urolithiasis. Plenum Press, New York, p 13
Pak CYC, Hayashi Y, Finlayson B, Chu S (1977) Estimation of state of saturation of brushite and calcium oxalate in urine and comparison of three methods. J Lab Clin Med 89:891
Robertson WG, Peacock M, Marshall RW, Marshall HD, Nordin BEC (1976) Saturation-inhibition index as a measure of the risk of calcium oxalate stone formation in the urinary tract. N Engl J Med 294:249
Sameull CT (1979) Uromucoid excretion in normal subjects, calcium stone formers and in patients with chronic renal failure. Urol Res 7:5
Tiselius HG, Almgard LE, Larson L, Sörba B (1978) A biochemical basis for grouping of patients with urolithiasis. Eur Urol 4:241
Will EJ, Bijvoot OLM, Blomen LJMJ, Vanderlinden H (1983) A seeded crystal growth method for measuring the effects of compounds or urine or the solubility, the growth and the agglomeration of calcium oxalate monohydrate crystals. J Crystal Growth 64:297
Tamm T, Horsfall FL (1950) Characterization and separation of an inhibitor of viral hemaglutination present in urine. Proc Soc Exp Med 74:108
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schnierle, P., Sialm, F., Seiler, H.G. et al. Investigations on macromolecular precipitation inhibitors of calcium oxalate. Urol. Res. 20, 7–11 (1992). https://doi.org/10.1007/BF00294327
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00294327