Summary
The plasmid pKM101 is known to protect Escherichia coli and Salmonella typhimurium against killing by far UV irradiation and to enhance UV-induced mutagenesis. The muc + gene of the plasmid is responsible for both of these effects. This paper shows that respiration of S. typhimurium shuts off about an hour after UV irradiation and that pKM101 prevents the shutoff. Plasmids which contained Tn5 translocatable elements, either in (and having produced a muc mutation) or flanking the muc + gene, have been introduced into S. typhimurium. The muc mutant plasmid, which does not protect its host against UV killing and does not enhance UV induced mutagenesis, also does not protect against UV induced respiration shutoff. Like-wise, plasmids in which the Tn5 translocatable elements flank the nuc + gene protect against shutoff of respiration. Thus the muc + gene of pKM101 is responsible for protection against UV induced shutoff of respiration in S. typhimurium.
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Communicated by B.A. Bridges
Research sponsored by the Office of Health and Environmental Research, U.S. Department of Energy, under contract W-7405-eng-26 with the Union Carbide Corporation and by the National Science Foundation under grant No. PCM 7908647 with the University of Tennessee, Knoxville
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Swenson, P.A. The muc+ gene of plasmid pKM101 prevents respiration shutoff in far ultraviolet-irradiated Salmonella typhimurium . Molec. Gen. Genet. 182, 422–425 (1981). https://doi.org/10.1007/BF00293930
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DOI: https://doi.org/10.1007/BF00293930