Summary
A modified histochemical method is described for the localization of creatine kinase by the technique of „incubation mixture film“. Rat skeletal muscle sections stained with Sjovall's method (1967) exhibited a staining pattern which appeared contrary to the biochemical data on the distribution of this enzyme. Localization of creatine kinase by the method described in this communication was obtained in both the broad white (Type II) and the narrow red (Type I) muscle fibres. The intensely staining sarcoplasm and sarcoplasmic reticulum in both fibre types suggests a distribution pattern for this enzyme which is more in keeping with the available biochemical data.
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Khan, M.A., Holt, P.G., Knight, J.O. et al. Incubation film technique for the histochemical localization of creatine kinase. Histochemie 26, 120–125 (1971). https://doi.org/10.1007/BF00293502
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DOI: https://doi.org/10.1007/BF00293502