Abstract
A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from −60 to +60 degrees. The reconstructed structure is about 3.0 μm long, 1.6 μm wide, and 0.8 μm thick. The mass distribution was fairly homogeneous within the chromatids and neither a hollow nor a dense core was observed. The distribution and course of fibers observed are most consistent with a looping model of chromosome structure.
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Harauz, G., Borland, L., Bahr, G.F. et al. Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs. Chromosoma 95, 366–374 (1987). https://doi.org/10.1007/BF00293184
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DOI: https://doi.org/10.1007/BF00293184