Die mutagene Wirkung von Endoxan bei der Maus

Abstract

• 1. Dosages of 420 mg and 210 mg Endoxan (Cyclophosphamid, Cytoxan) per kg body weigt were injected intraperitoneally into male C3H mice immediately before mating them with virgin females. The high dose led to sterility and to death of all males within the second week after treatment. The smaller quantity of Endoxan induced dominant lethal mutations, that is increased pre- and post-implantation death rates.

• 2. In order to record differences of sensitivity between the various stages of spermiogenesis of the mouse 10 males were treated with 210 mg or 60 mg Endoxan per kg and in 8 successive series mated with 30 virgin females (3 per male and series) for one week each. The statistical comparison of the dead zygotes of the Endoxan experiments with the control group showed, that all stages of the cycle of spermatogenesis were sensitive to the mutagenic action of Endoxan. The postmeiotic stages proved to be more sensitive to the mutagenic action of Endoxan than the premeiotic ones.