Summary
Mitochondrial (mt) and chloroplast (ct) DNAs from sugar beet carrying normal fertile and different cytoplasmic male sterile (cms) cytoplasms were compared by restriction analysis and for the occurrence of minicircles. One of the cms materials had the Owen cms cytoplasm currently used for hybrid production in sugar beet; the other three cms materials were derived from wild Beta beets. The mtDNAs from two of the latter cms types (C 7051, C 8640) differed from both the Owen and the fertile cytoplasms in fragment patterns seen after restriction enzyme analysis and in minicircle composition. The third cms type (C 8684) differed from the Owen cytoplasm in mini-circle composition, but restriction enzyme analysis revealed no differences. The presence of the different minicircles was confirmed by Southern hybridization using minicircle-specific clones. All bands hybridized as predicted by gel electrophoresis except a band in the cms type C 8640, which migrated in a similar manner as the c.c.c. form of the a minicircle. This band hybridized only faintly to a minicircle a-specific probe and could be removed by treatment with nuclease S1. In contrast to the large mtDNA variation, restriction analysis of ctDNA detected little variation between cytoplasms. The molecular characterization of the new sources of cms supports the results of previous crossings. Two of the cytoplasms are not only of independent origin, but are also most likely functionally different and thus may be of value in future production of hybrid sugar beet varieties.
Similar content being viewed by others
References
Bosemark NO (1979) Genetic poverty of the sugar beet in Europe. In: Zeven AC, Harten AM van (eds) Proc Conf Broadening Genet Base Crops. Pudoc, Wageningen, pp 29–35
Boutin V, Pannenbecker G, Ecke W, Schewe G, SaumitouLaprade P, Jean R, Vernet Ph, Michaelis G (1987) Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theor Appl Genet 73:625–629
Boutry M, Faber AM, Charbonnier M, Briquet M (1984) Microanalysis of plant mitochondrial protein synthesis products. Plant Mol Biol 3:445–452
Ellerström S (1963) Results from preliminary tests in Sweden of plants collected in the mediterranean region. Genet Agrar 17:200–220
Erickson L, Grant I, Beversdorf W (1986) Cytoplasmic male sterility in rapeseed (Brassica napus L.). 1. Restriction patterns of chloroplast and mitochondrial DNA. Theor Appl Genet 72:145–150
Goblet JP, Flamand MC, Briquet M (1985) A mitochondrial plasmid specifically associated with male sterility and its relation with other mitochondrial plasmids in Vicia faba L. Curr Genet 9:423–426
Hansen BM, Marcker KA (1984) DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugar beet mitochondria. Nucleic Acids Res 12:4747–4756
Kishima Y, Mikami T, Hirai A, Sugiura M, Kinoshita T (1987) Beta chloroplast genomes: analysis of Fraction I protein and chloroplast DNA variation. Theor Appl Genet 73:330–336
Levings III CS, Kim BD, Pring DR, Conde MF, Mans RJ, Laughnan JR, Gabay-Laughnan SJ (1980) Cytoplasmic reversion of cms-S in maize: association with a transpositional event. Science 209:1021–1023
Leroy P, Bazetoux S, Quétier F, Delbut J, Bervillé A (1985) A comparison between mitochondrial DNA of an isogenic male-sterile (S) and male-fertile (F) couple (HA 89) of sunflower. Curr Genet 9:245–251
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, New York, pp 109–112
Mikami T, Shinozaki K, Sugiura M, Kinoshita T (1984a) Characterization of chloroplast DNA from sugar beet with normal and male sterile cytoplasms. Jpn J Genet 59:497–504
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1984b) Chloroplast DNA diversity in the cytoplasms of sugar beet and its related species. Plant Sci Lett 36:231–235
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1985) Organelle genome diversity in sugar beet with normal and different sources of male sterile cytoplasms. Theor Appl Genet 71:166–171
Mikami T, Harada T, Kinoshita T (1986) Heterogeneity of circular mitochondrial DNA molecules from sugar beet with normal and male sterile cytoplasms. Curr Genet 10:695–700
Olsson G, Ellerström S (1957) Reseintryck från några Medelhavsländer. Sver Utsädesfören Tidskr 67:12–33
Owen FV (1945) Cytoplasmically inherited male-sterility in sugarbeets. J Agric Res 71:423–440
Palmer JD (1982) Physical and gene mapping of chloroplast DNA from Atriplex triangularis and Cucumis sativa. Nucleic Acids Res 10:1593–1605
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) An unusual mitochondrial DNA plasmid in the genus Brassica. Nature 301:725–728
Powling A (1981) Species of small DNA molecules found in mitochondria from sugar beet with normal and male sterile cytoplasms. Mol Gen Genet 183:82–84
Powling A (1982) Restriction endonuclease analysis of mitochondrial DNA from sugar beet with normal and malesterile cytoplasms. Heredity 49:117–120
Powling A, Ellis THN (1983) Studies on the organelle genomes of sugarbeet with male-fertile and male-sterile cytoplasms. Theor Appl Genet 65:323–328
Pring DR, Levings III CS (1978) Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89:121–136
Pring DR, Conde MF, Schertz KF (1982a) Organelle genome diversity in sorghum: Male-sterile cytoplasms. Crop Sci 22:414–421
Pring DR, Conde MF, Schertz KF, Levings III CS (1982b) Plasmid-like DNAs associated with mitochondria of cytoplasmic male-sterile sorghum. Mol Gen Genet 186:180–184
Thomas CM (1986) The nucleotide sequence and transcription of minicircular mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugarbeet. Nucleic Acids Res 14:9353–9370
Author information
Authors and Affiliations
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Halldén, C., Bryngelsson, T. & Bosemark, N.O. Two new types of cytoplasmic male sterility found in wild Beta beets. Theoret. Appl. Genetics 75, 561–568 (1988). https://doi.org/10.1007/BF00289121
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00289121