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Erfahrungen in der Anwendung der Ca-Phosphatmethode bei PH 7,5 zum Histochemischen Nachweis der Adenosintriphosphatase

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Summary

The use of the Ca-phosphate-method for the determination of ATP-ase pH 7.5 is reported. The results obtained in experimental myocardial infarction, autolysis, and in other lesion-producing mechanisms suggest, that the activity in question seems to be a mitochondrial ATP-ase, which by uncoupling the oxidative phosphorylation gives rise to an increased activity, that is detectable by histochemical means. It is pointed out, that the fixation of the sections is of importance. Our findings suggest that under certain conditions the Ca-phosphate-method is suitable for the determination of ATP-ase in a low pH range.

Zusammenfassung

Es wird über die Anwendung der Ca-Phosphatmethode zur histochemischen Darstellung der ATPase pH 7,5 berichtet. Ergebnisse beim experimentellen Herzinfarkt, bei der Autolyse sowie anderen Schädigungsmechanismen deuten darauf hin, daß es sich um den Nachweis einer mitochondrialen ATPase handelt, die bei Entkopplung der oxydativen Phosphorylierung der Anlaß für eine histochemisch nachweisbare Aktivitätssteigerung ist. Es wird auf die Bedeutung einer vorherigen Fixierung der Schnitte hingewiesen. Unsere Befunde lassen die Annahme zu, daß unter gewissen Voraussetzungen auch die Ca-Phosphatmethode zum Nachweis der ATPase im niederen pH-Bereich geeignet ist.

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Auszugsweise vorgetragen auf dem II. Internationalen Kongreß für Histo- und Cytochemie, Frankfurt a. M. vom 16.-21. 8. 64.

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Hecht, A. Erfahrungen in der Anwendung der Ca-Phosphatmethode bei PH 7,5 zum Histochemischen Nachweis der Adenosintriphosphatase. Histochemie 5, 116–124 (1965). https://doi.org/10.1007/BF00285503

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  • DOI: https://doi.org/10.1007/BF00285503

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