Abstract
The DST2 gene of Saccharomyces cerevisiae encodes a DNA strand exchange protein, STPβ, which is required for homologous recombination in both mitotic or meiotic cells. We have cloned a DST2-related gene from the fission yeast Schizosaccharomyces pombe and designated it dhp1 +. The nucleotide sequence of dhp1 + revealed an open reading frame encoding a protein composed of 991 amino acids. The predicted amino acid sequence was significantly homologous to the S. cerevisiae STPβ, but lacked the carboxy-terminal sequence present in STPβ. Furthermore, dhp1 + shows greater homology to RAT1/HKE1, a gene which is involved in RNA trafficking and processing. Genetic experiments showed that dhp1 + on an S. cerevisiae expression vector could rescue both the defects of the S. cerevisiae DST2 disruptant, slow growth rate and a sporulation defect, and the lethality of the S. cerevisiae rat1 ts mutation. This implies the functional similarity of dhp1 + to both DST2 and RAT1. However unlike DST2, dhp1 + is an essential gene for cell growth in S. pombe, suggesting that dhp1 + is not the true homologue of DST2 but rather of RAT1 in S. pombe. The possible roles of dhp1 + in recombination and cell growth in S. pombe are discussed.
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Amberg DC, Goldstein AL, Cole CN (1992) Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA. Genes Dev 6:1173–1189
Arai N, Kawasaki K, Shibata T (1992) A multicomponent protein of a fission yeast that promotes joint molecule formation from homologous DNAs. J Biol Chem 267:3514–3522
Baker JM (1992) Is DNA strand transfer protein also a transcription factor? Cell 70:877
Clark AB, Dykstra CC, Sugino A (1991) Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein. Mol Cell Biol 11:2576–2582
Cox MM, Lehman IR (1987) Enzymes of general recombination. Annu Rev Biochem 56:229–262
DeVeaux LC, Hoagland NA, Smith GR (1992) Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe. Genetics 130:251–262
Dykstra CC, Hamatake RK, Sugino A (1990) DNA strand transfer protein β (STPβ) from yeast mitotic cells differs from strand transfer protein α from meiotic cells. J Biol Chem 265:10968–10973
Dykstra CC, Kitada K, Clark AB, Hamatake RK, Sugino A (1991) Cloning and characterization of DST2: the gene for DNA strand transfer protein β from Saccharomyces cerevisiae. Mol Cell Biol 11:2583–2592
Erdman VA, Huysmans E, Vanderberghe A, de Wachter R (1983) Collection of published 5S and 5.8S ribosomal RNA sequences. Nucleic Acids Res 11:105–133
Gomez-Marquez J, Segade F (1988) Prothymosin α is a nuclear protein. FEBS Lett 226:217–219
Gutz H, Heslot H, Leupold V, Loprieno N (1974) In: King RC (ed) Schizosaccharomyces pombe, vol 1. Plenum Press, New York, pp 395–446
Gysler-Junker A, Bodi Z, Kohli J (1991) Isolation and characterization of Schizosaccharomyces pombe mutants affected in mitotic recombination. Genetics 128:495–504
Holland JP, Holland MJ (1979) The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae. J Biol Chem 254:9838–9845
Holliday R (1964) A mechanism for gene conversion in fungi. Genet Res 5:282–303
Johnson AW, Kolodner RD (1991) Strand exchange protein 1 from Saccharomyces cerevisiae. J Biol Chem 266:14046–14054
Kenna M, Stevens A, McCammon M, Douglas MG (1993) An essential yeast gene with homology to the exonuclease-encoding XRN1/KEM1 gene also encodes a protein with exoribonuclease activity. Mol Cell Biol 13:341–350
Kim J, Ljungdahl, Fink GR (1990) kem mutations affect nuclear fusion in Saccharomyces cerevisiae. Genetics 126:799–812
Kipling D, Tambini C, Kearsey SE (1991) rar mutations which increase artificial chromosome stability in Saccharomyces cerevisiae identify transcription and recombination proteins. Nucleic Acids Res 19:1385–1391
Kolodner K, Evans DH, Morrison PT (1987) Purification and characterization of an activity from Saccharomyces cerevisiae that catalyzes homologous pairing and strand exchange. Proc Natl Acad Sci USA 84:5560–5564
Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
Larimer FW, Stevens A (1990) Disruption of the gene XRN1, coding for a 5′-3′ exoribonuclease, restricts yeast cell growth. Gene 95:85–90
Lin Y, Larson KL, Dorer R, Smith GR (1992) Meiotically induced rec7 and rec8 genes of Schizosaccharomyces pombe. Genetics 132:75–85
Meselson MS, Radding CM (1975) A general model for genetic recombination. Proc Natl Acad Sci USA 73:300–304
Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194:795–823
Mount S (1982) A catalogue of splice junction sequences. Nucleic Acids Res 10:459–472
Ponticelli AS, Smith GR (1989) Meiotic recombination-deficient mutants of Schizosaccharomyces pombe. Genetics 123:45–54
Radding CM (1982) Homologous pairing and strand exchange in genetic recombination Annu Rev Genet 16:405–437
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schmidt H, Kapitza P, Gutz H (1987) Switching genes in Schizosaccharomyces pombe: their influence on cell viability and recombination. Curr Genet 11:303–308
Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics: laboratory course manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sugino A, Nittiss J, Resnick MA (1988) ATP-independent DNA strand transfer catalyzed by protein(s) from meiotic cells of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci USA 85:3683–3687
Szostak JW, Rothenstein RJ, Stahl FW (1983) The double-strandbreak repair model for recombination. Cell 33:25–35
Tishkoff DX, Johnson AW, Kolodner RD (1991) Molecular and genetic analysis of the gene encoding the Saccharomyces cerevisiae strand exchange protein Sep1. Mol Cell Biol 11:2593–2608
Vieira J, Messing J (1987) Production of single-stranded plasmid DNA. Methods Enzymol 153:3–11
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Communicated by M. Sekiguchi
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Sugano, S., Shobuike, T., Takeda, T. et al. Molecular analysis of the dhp1 + gene of Schizosaccharomyces pombe: an essential gene that has homology to the DST 2 and RAT 1 genes of Saccharomyces cerevisiae . Molec. Gen. Genet. 243, 1–8 (1994). https://doi.org/10.1007/BF00283869
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DOI: https://doi.org/10.1007/BF00283869