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Molecular cloning of the UMP synthase gene rudimentary-like from Drosophila melanogaster

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Summary

The rudimentary-like locus encodes UMP synthase, a bienzyme protein containing the last two enzyme activities of de novo pyrimidine biosynthesis: orotate phosphoribosyltransferase and orotidylate decarboxylase. This locus lies within chromosome region 9313. New mutations have been used to refine the 9313 cytogenetic map and a chromosome walk has been executed to clone DNA from this region. DNA encoding UMP synthase was identified using mixed oligonucleotides which were based on sequences derived from conserved peptide tracts of the protein in other species. cDNA clones of the embryonic UMP synthase mRNA have been isolated and used to define the extent of genomic DNA sequences which encode the transcript. The embryonic RNA is approximately 1.75 kb in length.

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References

  • Bender W, Spierer P, Hogness D (1983) Chromosome walking and jumping to isolate DNA from the bithorax complex and the Ace and rosy loci in Drosophila melanogaster. J Mol Biol 168:17–33

    Google Scholar 

  • Chirgwin J, Przbyla A, MacDonald R, Rutter W (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299

    Google Scholar 

  • Davis L, Dibner M, Battey J (1986) Basic Methods in Molecular Biology. Elsevier, New York

    Google Scholar 

  • Falk D, Nash D (1974) Pyrimidine auxotrophy in Drosophila. Normal-winged, auxotrophic mutants and dominant auxotrophy at the rudimentary locus. Mol Gen Genet 131:339–349

    Google Scholar 

  • Fausto-Sterling A, Hsieh L (1976) Studies on the female-sterile mutant rudimentary of Drosophila melanogaster. I. An analysis of the rudimentary wing phenotype. Dev Biol 51:269–281

    Google Scholar 

  • Garbe J, Pardue M (1986) Heat shock locus 93D of Drosophila melanogaster: a spliced RNA most strongly conserved in the intron sequence. Proc Natl Acad Sci USA 83:1812–1816

    Google Scholar 

  • Jacquet M, Guilbaud R, Kessin R (1988) Sequence analysis of the DdPYR5-6 gene coding for UMP synthase in Dictyostelium discoideum and comparison with orotate phosphoribosyltransferases and OMP decarboxylase. Mol Gen Genet 211:441–445

    Google Scholar 

  • Jarry B, Falk D (1974) Functional diversity within the rudimentary locus of Drosophila melanogaster. Mol Gen Genet 135:113–122

    Google Scholar 

  • Jones M (1980) Pyrimidine nucleotide biosynthesis in animals: Genes, enzymes and regulation of UMP biosynthesis. Annu Rev Biochem 49:253–279

    Google Scholar 

  • Jones W, Rawls J (1988) Genetic and molecular mapping of chromosome region 85A in Drosophila melanogaster. Genetics 120:733–742

    Google Scholar 

  • Kanalas J, Hutton J, Suttle D (1985) Characterization of pyrazofurin-resistant HeLa cells with amplification of UMP synthase gene. Somatic Cell Mol Genet 11:359–369

    Google Scholar 

  • Lewis E (1960) A new standard food medium. Drosophila Inf Serv 34:117

    Google Scholar 

  • Lewis E, Bacher F (1968) Method of feeding ethylmethyl-sulfonate to Drosophila males. Drosophila Inf Serv 43:193

    Google Scholar 

  • Lindsley D, Grell E (1968) Genetic Variations of Drosophila melanogaster. Carnegie Inst Wash Pub 627

  • Maniatis T, Hardison R, Lacy E, Lauer J, O'Connell C, Quon D, Sim D, Efstratiadis A (1978) The isolation of structural genes from libraries of eucaryotic DNA. Cell 15:687–701

    Google Scholar 

  • Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • McClard R, Black M, Livingstone L, Jones M (1980) Isolation and initial characterization of the single polypeptide that synthesizes uridine-5′-monophosphate from orotate in Ehrlich ascites carcinoma. Biochemistry 19:4699–4706

    Google Scholar 

  • Mohler J, Pardue M (1984) Mutational analysis of the region surrounding the 93D heat shock locus of Drosophila melanogaster. Genetics 106:249–265

    Google Scholar 

  • Norby S (1970) A specific nutritional requirement for pyrimidines in rudimentary mutants of Drosophila melanogaster. Hereditas 66:205–214

    Google Scholar 

  • Okada M, Kleinman I, Schneiderman H (1974) Repair of a genetically-caused defect in oogenesis in Drosophila melanogaster by transplantation of cytoplasm from wild-type eggs and by injection of pyrimidine nucleosides. Dev Biol 37:55–62

    Google Scholar 

  • Poole S, Kauvar L, Drees B, Kornberg T (1985) The engrailed locus of Drosophila: structural analysis of the embryonic transcript. Cell 40:37–43

    Google Scholar 

  • Rawls J (1979) The enzymes for de novo pyrimidine biosynthesis in Drosophila melanogaster: their localization, properties and expression during development. Comp Biochem Physiol [B] 62:207–216

    Google Scholar 

  • Rawls J (1980) Identification of a small genetic region that encodes orotate phosphoribosyltransferase and orotidylate decarboxylase in Drosophila melanogaster. Mol Gen Genet 178:43–49

    Google Scholar 

  • Rawls J (1981) Genetic complementation and enzyme correlates at the locus encoding the last two steps of de novo pyrimidine biosynthesis in Drosophila melanogaster. Mol Gen Genet 184:174–179

    Google Scholar 

  • Rawls J, Fristrom J (1975) A complex genetic locus that controls the first three steps of pyrimidine biosynthesis in Drosophila. Nature 255:738–739

    Google Scholar 

  • Rawls J, Chambers C, Cohen W (1981) A small genetic region that controls dihydro-orotate dehydrogenase in Drosophila melanogaster. Biochem Genet 19:115–127

    Google Scholar 

  • Rigby P, Deickman M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J Biol Chem 113:237–252

    Google Scholar 

  • Rose M, Grisafi P, Botstein D (1984) Structure and function of the yeast URA3 gene: expression in Escherichia coli. Gene 29:113–124

    Google Scholar 

  • Sanger F, Nicklen S, Coulson A (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Steller H, Pirrotta V (1985) A transposable P vector that confers selectable G418 resistance to Drosophila larvae. EMBO J 4:167–171

    Google Scholar 

  • Suttle P (1983) Increased levels of UMP synthase protein and mRNA in pyrazofurin-resistant rat hepatoma cells. J Biol Chem 258:7707–7713

    Google Scholar 

  • Suttle P, Bugg B, Winkler J, Kanalas J (1988) Molecular cloning and nucleotide sequence for the complete coding region of human UMP synthase. Proc Natl Acad Sci USA 85:1754–1758

    Google Scholar 

  • Vincent T (1986) Genetic analysis of the rudimentary-like region in Drosophila melanogaster. MSc dissertation, University of Kentucky, Kentucky USA

    Google Scholar 

  • Wood W, Gitschier J, Lasky L, Lawn R (1985) Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci USA 82:1585–1588

    Google Scholar 

  • Yanisch-Perrson C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119

    Google Scholar 

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Author notes

  1. Marcia Eisenberg

    Present address: Laboratory of Molecular Genetics, NIEHS, Research Triangle Park, NC, USA

  2. Karen Gathy

    Present address: Department of Biochemistry, University of Kentucky, Lexington, Kentucky, USA

Authors and Affiliations

  1. T.H. Morgan School of Biological Sciences, Molecular and Cell Biology Group, University of Kentucky, 40506, Lexington, KY, USA

    Marcia Eisenberg, Karen Gathy, Teresa Vincent & John Rawls

Authors
  1. Marcia Eisenberg
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  2. Karen Gathy
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  3. Teresa Vincent
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  4. John Rawls
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Communicated by B.H. Judd

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Eisenberg, M., Gathy, K., Vincent, T. et al. Molecular cloning of the UMP synthase gene rudimentary-like from Drosophila melanogaster . Molec. Gen. Genet. 222, 1–8 (1990). https://doi.org/10.1007/BF00283015

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  • DOI: https://doi.org/10.1007/BF00283015

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