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Regulation of the yeast RAD2 gene DNA damage-dependent induction correlates with protein binding to regulatory sequences and their deletion influences survival

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Summary

In the yeast Saccharomyces cerevisiae the RAD2 gene is absolutely required for damage-specific incision of DNA during nucleotide excision repair and is inducible by DNA-damaging agents. In the present study we correlated sensitivity to killing by DNA-damaging agents with the deletion of previously defined specific promoter elements. Deletion of the element DRE2 increased the UV sensitivity of cells in both the G1/early S and S/G2 phases of the cell cycle as well as in stationary phase. On the other hand, increased UV sensitivity associated with deletion of the sequence-related element DRE1 was restricted to cells irradiated in G1/S. Specific binding of protein(s) to the promoter elements DRE1 and DRE2 was observed under non-inducing conditions using gel retardation assays. Exposure of cells to DNA-damaging agents resulted in increased protein binding that was dependent on de novo protein synthesis.

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Authors and Affiliations

  1. Laboratory of Molecular Pathology, Department of Pathology, The University of Texas Southwestern Medical Center at Dallas, 75235, Dallas, TX, USA

    Wolfram Siede & Errol C. Friedberg

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  1. Wolfram Siede
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  2. Errol C. Friedberg
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Communicated by R. Devoret

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Siede, W., Friedberg, E.C. Regulation of the yeast RAD2 gene DNA damage-dependent induction correlates with protein binding to regulatory sequences and their deletion influences survival. Molec. Gen. Genet. 232, 247–256 (1992). https://doi.org/10.1007/BF00280003

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  • DOI: https://doi.org/10.1007/BF00280003

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