Skip to main content
SpringerLink
Log in

Identification, properties and genetic control of UDP-glucose: Isovitexin 7-O-glucosyltransferase isolated from petals of Melandrium album

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

In extracts of petals of M. album, an enzyme has been demonstrated which catalyzes the transfer of the glucosyl moiety of UDP-glucose to the 7-hydroxylgroup of isovitexin.

This enzyme is controlled by a dominant gene G; in plants with the recessive genotype no glucosyltransferase activity could be detected.

The enzyme was purified 16-fold by (NH4)2SO4 fractionation and Sephadex-chromatography.

The glucosyltransferase had a pH optimum of pH 7.5, was not stimulated by divalent metal ions, and had a “true Km” value of 1.2x10-4 M for UDP-glucose and a “true Km” value of 4.6x10-4 M for isovitexin.

Several flavones with an apigenin hydroxylation pattern could serve as glucosyl acceptors. The highest activity was found, however, with isovitexin.

The enzyme was unable to catalyze the transfer of xylose from UDP-xylose to the 7-hydroxylgroup of isovitexin, although isovitexin 7-O-xyloside has been found in petals of M. album plants.

ADP-glucose could not serve as a glucosyl donor.

The transferase activity was also present in leaves and calyces of GG plants. In these organs the transferase uses another flavone as a substrate. Neither isovitexin 7-O-glucoside nor isovitexin could be detected in these organs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andrews, P.: The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J. 96, 595–606 (1965).

    Google Scholar 

  • Barber, G. A.: Enzymic glycosylation of quercetin to rutin. Biochemistry 1, 463–468 (1962).

    Google Scholar 

  • Barber, G. A., Chang, M. T. Y.: The enzymic glycosylation of quercetin to quercitrin. Phytochem. 7, 35–39 (1968).

    Google Scholar 

  • Brederode, J. van, Nigtevecht, G. van: The genetic control of isovitexin-glycosylation in the petals of M. album. Molec. gen. Genet. 118, 247–259 (1972).

    Google Scholar 

  • Florini, J. R., Vestling, C. S.: Graphical determination of the dissociation constants for two-substrate enzyme systems. Biochim. biophys. Acta (Amst.) 25, 575–578 (1957).

    Google Scholar 

  • Galston, A. W.: Flavonoids and photomorphogenesis in peas. In: Harborne, J. B., Swain, T. (eds.), Perspectives in phytochemistry, p. 193–204. London-New York: Academic Press 1969.

    Google Scholar 

  • Hagen, C. W.: The differentiation of pigmentation in flowerparts: II. Changes in pigments during developments of buds in Impatiens balsamina genotype llHHPrPr. Amer. J. Bot. 53, 54–60 (1966).

    Google Scholar 

  • Harborne, J. B.: Phenolic glycosides and their natural distribution: V. Natural occurrence. In: Harborne, J. B. (ed.), Biochemistry of phenolic compounds, p. 152–165. London-New York: Academic Press, 1964.

    Google Scholar 

  • Larson, R. L.: Glucosylation of quercetin by a maize pollen enzyme. Phytochem. 10, 3073–3076 (1971).

    Google Scholar 

  • Larson, R. L., Lonergan, C. M.: Glucosyltransferase activity in water extract of maize pollen. Planta (Berl.) 103, 361–364 (1972).

    Google Scholar 

  • Levin, D. A.: Plant phenolics: an ecological perspective. Amer. Naturalist 105, 157–181 (1971).

    Google Scholar 

  • Lineweaver, H., Burk, D.: The determination of enzyme dissociation constants. J. Amer. chem. Soc. 56, 658–666 (1934).

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).

    Google Scholar 

  • Nigtevecht, G. van: Genetic studies in dioecious Melandrium. I. Sex-linked and sex influenced inheritance in Melandrium album and Melandrium dioicum. Genetica 37, 281–306 (1966).

    Google Scholar 

  • Ortman, R., Sandermann, H., Jr., Grisebach, H.: Transfer of apiose from UDP-apiose to 7-O-(beta-D-glucosyl)-apigenin and 7-O-(beta-D-glucosyl)-chrysoeriol with an enzyme preparation from parsley. FEBS Letters 7, 164–166 (1970).

    Google Scholar 

  • Sutter, A., Ortman, R., Grisebach, H.: Purification and properties of an enzyme from cell suspension cultures of parsley catalyzing the transfer of D-glucose from UDP-D-glucose to flavonoids. B.B.A. 258, 71–81 (1972).

    Google Scholar 

  • Weissenböck, G., Reznik, H.: Änderungen des Flavonoid-Musters während der Samenkeimung von Impatiens balsamina L. Z. Pflanzenphysiol. 63, 114–129 (1970).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Genetical Institute, University of Utrecht, Utrecht, The Netherlands

    J. van Brederode & G. van Nigtevecht

Authors
  1. J. van Brederode
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. van Nigtevecht
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Stubbe

Rights and permissions

Reprints and permissions

About this article

Cite this article

van Brederode, J., van Nigtevecht, G. Identification, properties and genetic control of UDP-glucose: Isovitexin 7-O-glucosyltransferase isolated from petals of Melandrium album . Molec. Gen. Genet. 122, 215–229 (1973). https://doi.org/10.1007/BF00278598

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00278598

Keywords

Search

Navigation