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Cloning and expression of distinct nitrite reductases in tobacco leaves and roots

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Summary

Three tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR cDNA as a probe. Sequence analysis and Southern blot hybridization revealed four genes in tobacco. Two of these genes presumably derived from the ancestral species Nicotiana tomentosiformis, the other two from the ancestor N. sylvestris. Northern blot analysis showed that one gene from each ancestral genome was expressed predominantly in leaves, whilst RNA from the other was detected mostly in roots. The accumulation of both leaf and root NiR mRNAs was induced by nitrate and repressed by nitrate- or ammonium-derived metabolites. In addition, the expression of the root NiR gene was detectable in leaves of a tobacco nitrate reductase (NR)-deficient mutant. Thus, the regulation of expression of tobacco NiR genes is comparable to the regulation of expression of barley NR genes.

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References

  • Back E, Burkhart W, Moyer M, Privalle L, Rothstein S (1988) Isolation of cDNA clones coding for spinach nitrite reductase: complete sequence and nitrate induction. Mol Gen Genet 212:20–26

    Google Scholar 

  • Bourgin JP, Chapeau Y, Missonier C (1979) Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol Plant 45:288–292

    Google Scholar 

  • Caboche M, Rouzé P (1991) Nitrate reductase: a target for molecular and cellular studies in higher plants. Trends Genet 6:187–192

    Google Scholar 

  • Calza R, Huttner E, Vincentz M, Rouzé P, Galangau F, Vaucheret H, Chérel I, Meyer C, Kronenberger J, Caboche M (1987) Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductases from higher plants. Mol Gen Genet 309:552–562

    Google Scholar 

  • Cheng C, Dewdney J, Kleinhofs A, Goodman H (1986) Cloning and nitrate induction of nitrate reductase mRNA. Proc Natl Acad Sci USA 83:6825–6828

    Google Scholar 

  • Cheng C, Dewdney J, Nam H, den Boer B, Goodman H (1988) A new locus (NIA1) in Arabidopsis thaliana encoding nitrate reductase. EMBO J 7:3309–3314

    Google Scholar 

  • Choi H, Kleinhofs A, An G (1991) Nucleotide sequence of rice nitrate reductase genes. Plant Mol Biol 13:731–733

    Google Scholar 

  • Crawford N, Campbell W, Davis R (1986) Nitrata reductase from squash: cDNA cloning and nitrate regulation. Proc Natl Acad Sci USA 83:8073–8076

    Google Scholar 

  • Crawford N, Smith M, Bellissimo D, Davis R (1988) Sequence and nitrate regulation of the Arabidopsis thaliana mRNA encoding nitrate reductase, a metalloflavoprotein with three functional domains. Proc Natl Acad Sci USA 85:5006–5010

    Google Scholar 

  • Daniel-Vedele F, Dorbe MF, Caboche M, Rouzé P (1989) Cloning and analysis of the tomato nitrate reductase-encoding gene: protein domain structure and amino-acid homologies in higher plants. Gene 85:371–380

    Google Scholar 

  • Faure JD, Vincentz M, Kronenberger J, Caboche M (1991) Coregulated expression of nitrate and nitrite reductases. Plant J 1:107–113

    Google Scholar 

  • Friemann A, Brinkmann K, Hachtel W (1992) Sequence of a cDNA encoding nitrite reductase from the tree Betula pendula and identification of conserved protein regions. Mol Gen Genet 231:411–416

    Google Scholar 

  • Galangau F, Daniel-Vedele F, Moureaux T, Dorbe MF, Leydecket MT, Caboche M (1988) Expression of leaf nitrate reductases genes from tomato and tobacco in relation to light-dark regimes and nitrate supply. Plant Physiol 88:383–388

    Google Scholar 

  • Gowri G, Campbell W (1989) cDNA clones for corn leaf NADH: nitrate reductase and chloroplast NAD(P): glyceraldehyde-3-phosphate dehydrogenase. Characterization of the clones and analysis of the expression of the genes in leaves as influenced by nitrate in the light and dark. Plant Physiol 90:792–798

    Google Scholar 

  • Kleinhofs A, Kuo, Warner R (1980) Characterisation of nitrate reductase-deficient barley mutants. Mol Gen Genet 177:421–425

    Google Scholar 

  • Lanhers K, Kramer V, Back E, Privalle L, Rothstein S (1988) Molecular cloning of complementary DNA encoding maize nitrite reductase. Molecular analysis and nitrate induction. Plant Physiol 88:741–746

    Google Scholar 

  • Lee K, Townsend J, Tepperman J, Black M, Chui C, Mazur B, Dunsmuir P, Bedbrook J (1988) The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J 7:1241–1248

    Google Scholar 

  • Miyazaki J, Juricek M, Angelis K, Schnorr K, Kleinhofs A, Warner R (1991) Characterization and sequence of a novel nitrate reductase from barley. Mol Gen Genet 228:329–334

    Google Scholar 

  • Müller A (1983) Genetic analysis of nitrate reductase-deficient tobacco plants regenerated from mutants cells: evidence for duplicate structural genes. Mol Gen Genet 192:275–281

    Google Scholar 

  • Müller A, Grafe R (1978) Isolation and characterisation of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76

    Google Scholar 

  • Pelsy F, Kronenberger J, Pollien JM, Caboche M (1991) M2 seed screening for nitrate reductase deficiency in Nicotiana plumbaginifolia. Plant Sci 76:109–114

    Google Scholar 

  • Prosser I, Lazarus C (1990) Nucleotide sequence of a spinach nitrate reductase cDNA. Plant Mol Biol 15:187–190

    Google Scholar 

  • Vaucheret H, Vincentz M, Kronenberger J, Rouzé P, Caboche M (1989) Molecular cloning and characterization of the two homeologous genes coding for nitrate reductase in tobacco. Mol Gen Genet 216:10–15

    Google Scholar 

  • Vaucheret H, Kronenberger J, Lépingle A, Vilaine F, Boutin JP, Caboche M (1992) Inhibition of tobacco nitrite reductase activity by antisense RNA. Plant J (in press)

  • Warner R, Narayanan K, Kleinhofs A (1987) Inheritance and expression of NAD(P)H nitrate reductase in barley. Theor Appl Genet 74:714–717

    Google Scholar 

  • Wray J, Kinghorn J (1989) Molecular and genetic aspects of nitrate assimilation. Oxford Science Publications, Oxford

    Google Scholar 

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Communicated by R. Hagemann

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Kronenberger, J., Lepingle, A., Caboche, M. et al. Cloning and expression of distinct nitrite reductases in tobacco leaves and roots. Molec. Gen. Genet. 236, 203–208 (1993). https://doi.org/10.1007/BF00277113

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  • DOI: https://doi.org/10.1007/BF00277113

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