Summary
We have recently cloned both the bovine protamine (Krawetz et al. 1987, DNA 6: 47–57) and high mobility group (HMG-1) cDNAs (Pentecost and Dixon 1984, Bioscience Reports 4: 49–57). They have been used as probes for Restriction Fragment Length Polymorphism analysis of male-female pairs of different species and breeds, within the genus Bos. Utilizing this approach we have studied inheritance, chromosomal location and gene copy number of the bovine protamine and HMG-1 genes. This revealed that these nuclear protein genes are highly conserved suggesting that selective pressure has maintained their gene structures during evolution. A polymorphic Taq 1 restriction fragment was identified that was shown to be a heritable marker. These genes are not sex-linked and are present in a single copy for protamine and at least two copies for the HMG-1.
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Krawetz, S.A., Bricker, R.A., Connor, W. et al. Restriction fragment length polymorphism (RFLP) analysis of bovine nuclear protein genes. Theoret. Appl. Genetics 75, 402–409 (1988). https://doi.org/10.1007/BF00276742
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DOI: https://doi.org/10.1007/BF00276742