Summary
Mitochondria isolated from etiolated shoots of a range of maize genotypes with the “Texas” cytoplasm conferring cytoplasmically-inherited male sterility, are sensitive to a pathotoxin isolated from Helmintho-sporium maydis, race T. The pathotoxin inhibits oxidation of α ketoglutarate and malate and stimulates NADH oxidation. The time taken for the pathotoxin to induce these changes is a measure of the sensitivity of the mitochondria to the pathotoxin. A range of nine different pairs of genotypes, each pair differing principally in the presence of nuclear male fertility restorer alleles has been compared in their sensitivity to pathotoxin. In every case the line carrying the restorer alleles is more resistant to the pathotoxin. The restored genotypes can be quantitatively arranged into groups which correspond to the four different sources of the restorer genes in these lines. It is suggested that the restorer genes cause changes in mitochondria, which modify the functional aberration introduced by the cytoplasmically-inherited mutation causing sterility.
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Literature
Arntzen, C. J., Koeppe, D. E., Miller, R. J., Peverly, J. H.: The effect of pathotoxin from Helminthosporium maydis (race T) on energy-linked processes of corn seedlings. Physiol. Plant Path. 3, 79–89 (1973).
Beckett, J. B.: Classification of male sterile cytoplasms in maize (Zea mays L.). Crop Sci. 11, 724–726 (1971).
Berquist, R. R., Peverly, G.: Reaction of corn inbreds and hybrids with different cytoplasms and genotypes to Helminthosporium maydis-Race T. Plant Disease Reporter 56, 112–114 (1972).
Bianchi, F.: Transmission of male sterility in Petunia by grafting. Genen Phaenen 8, 53–64 (1963).
Curtis, G. J.: Graft transmission of male sterility in sugar beet (Beta vulgaris L.) Euphytica 16, 419–424 (1967).
De Vries, A. P., Ie, T. S.: Electron-microscopy of anther tissue and pollen of male sterile and fertile wheat (Triticum aestivum L.). Euphytica 19, 103–120 (1970).
Duvick, D. N.: Cytoplasmic pollen sterility in corn. Adv. in Genetics 13, 1–56 (1965).
Edwardson, J. R.: Cytoplasmic male sterility. Bot. Rev. 36, 341–420 (1970).
Edwardson, J. R., Corbett, M. K.: Asexual transmission of cytoplasmic male sterility. Proc. Nat. Acad. Sci. 47, 390–396 (1961).
Flavell, R. B.: The inhibition of electron transport by Helminthosporium maydis race T pathotoxin in maize mitochondria altered by a cytoplasmically-inherited mutation. Physiol. Plant Pathol. (1974a, in press).
Flavell, R. B.: A model for the mechanism of cytoplasmic male sterility, with special reference to maize. Plant Sci. Letters 3, 259–263 (1974b).
Frankel, R.: Graft induced transmission to progeny of cytoplasmic male sterility in Petunia. Science 124, 684–685 (1956).
Frankel, R.: Further evidence on graft induced transmission to progeny of cytoplasmic male sterility in Petunia. Genetics 47, 641–646 (1962).
Frankel, R.: The nature of cytoplasmic male sterility in Petunias. (English summary). Spec. Bull. 69, Nat. and Univ. Inst. Agric. Rehovot 1-VIII (1964).
Gracen, V. E., Forster, M. J., Grogan, C. O.: Reactions of corn (Zea mays) genotypes and cytoplasms to Helminthosporium maydis toxin. Plant Disease Reporter 55, 938–941 (1971).
Gracen, V. E., Grogan, C. O.: Reactions of corn (Zea mays) seedlings with non sterile, Texas male sterile, and restored Texas male sterile cytoplasms to Helminthosporium maydis toxin. Plant Disease Reporter 56, 432–433 (1972).
Gracen, V. E., Grogan, C. O., Forster, M. J.: Permeability changes induced by Helminthosporium maydis, race T, toxin. Canad. J. of Botany 50, 2167–2170 (1972).
Hilty, J. W., Josephson, L. M.: Reaction of corn inbreds with different cytoplasms to Helminthosporium maydis. Plant Disease Reporter 55, 195–198 (1971).
Hooker, A. L.: Helminthosporium maydis a challenge for the maize breeder. Proceedings of the 6th meeting of the Maize and Sorghum section of Eucarpia 333–367 (1971).
Hooker, A. L., Smith, D. R., Lim, S. M., Beckett, J. B.: Reaction of corn seedlings with male sterile cytoplasm to Helminthosporium maydis. Plant Disease Reporter 54, 708–712 (1970a).
Hooker, A. L., Smith, D. R., Lim, S. M., Musson, M. D.: Physiological races of Helminthosporium maydis and disease resistance. Plant Disease Reporter 54, 1109 to 1110 (1970b).
Miller, R. J., Koeppe, D. E.: Southern corn leaf blight: susceptible and resistant mitochondria. Science 173, 67–69 (1971).
Mitchell, M. B., Mitchell, H. K.: A nuclear gene suppressor of a cytoplasmically inherited character in Neurospora crassa. J. Gen. Microbiol. 14, 84–89 (1956).
Nass, S.: Similarities of bacteria and mitochondria. Intern. Rev. Cytology 25, 55–129 (1969).
Ohta, Y.: Effect of thermoshock and virus inoculation on cytoplasmic male sterility in Capsium and Petunia. Ann. Rep. Nat. Inst. Genet. Japan 18, 26 (1967).
Peterson, P. A., Flavell, R. B., Barratt, D. H. P.: Altered mitochondrial membrane activities associated with cytoplasmically-inherited disease sensitivity in maize. Theor. Appl. Genetics 45, 309–314 (1975).
Rhoades, M. M.: Gene induced mutation of a heritable cytoplasmic factor producing male sterility in maize. Proc. Nat. Acad. Sci. US. 36, 634–635 (1950).
Russell, W. A., Marquez-Sanchez, F.: Effect of cytoplasmic male sterility and restorer genes on performance among different genotypes of corn (Zea mays L.) Crop Sci. 6, 294–295 (1966).
Sager, R.: Cytoplasmic genes and organelles. New York: Academic Press 1972.
Smith, D. R., Hooker, A. L., Lim, S. M., Beckett, J. B.: Disease reaction of thirty sources of cytoplasmic male sterile corn to Helminthosporium maydis race T. Crop Sci. 11, 772–773 (1971).
Srivastava, H. K., Sarkissian, I. V., Shands, H. L.: Mitochondrial complementation and cytoplasmic male sterility in wheat. Genetics 63, 611–618 (1969).
Taylor, D. L.: Chloroplasts as symbiotic organelles. Intern. Rev. Cytology 27, 29–64 (1970).
Tipton, C. L., Mondal, M. H., Uhlig, J.: Inhibition of the K+ stimulated ATPase of maize root microsomes by Helminthosporium maydis race T pathotoxin. Biochem. and Biophys. Res. Comm. 51, 725–728 (1973).
Turbin, N. V., Atrashenok, N. V., Palilova, A. M., Lyulkina, E. I.: Mitochondrial structure in ovules of hybrid corn obtained by crossing sterile lines with fertility restoring lines. Doklady Biol. Sci. 182, 536–538 (1968).
Turner, M. T., Martinson, C. A.: Susceptibility of corn lines to Helminthosporium maydis toxin. Plant Disease Reporter 56, 29–32 (1972).
Ullstrup, A. J.: The impacts of the southern corn leaf blight epidemics of 1970–1971. Ann. Rev. Phytopathology 10, 37–50 (1972).
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Communicated by R. Riley
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Barratt, D.H.P., Flavell, R.B. Alterations in mitochondria associated with cytoplasmic and nuclear genes concerned with male sterility in maize. Theoret. Appl. Genetics 45, 315–321 (1975). https://doi.org/10.1007/BF00276686
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DOI: https://doi.org/10.1007/BF00276686