Summary
The fusion of plant protoplasts is greatly enhanced by a rise in temperature in the presence of the fusion-inducing agents polyethylene glycol or polyvinyl alcohol. Kinetic analysis of this reaction reveals that membrane fluidity is important in regulating the fusion process.
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Literature
Ahkong, Q.F.; Cramp F.C.; Fisher, D.; Howell, J.I.; Tampion, W.; Verrinder, M.; Lucy, J.A. (1973): Chemically-induced and thermally-induced cell fusion: Lipid-lipid interactions. Nature 242, 215–217
Ahkong, Q.F.; Tampion, W.; Lucy, J.A. (1975): Promotion of cell fusion by divalent cation ionophores. Nature 256, 208–209
Keller, W.A.; Melchers, G. (1974): The effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28c, 737–741
Moelwyn-Hughes, E.A. (1961): Physical Chemistry. 2nd ed., pp. 1304–1305. Oxford: Pergamon Press
Nagata, T. (1978): A novel cell-fusion method of protoplasts by polyvinyl alcohol. Z. Naturwiss. 65, 263–264
Okada, Y. (1976): Cell Fusion and Cell Engineering. (in Japanese), p. 22. Tokyo: Kodansha
Poste, G. (1974): Mechanisms of virus induced cell fusion. Intern. Rev. Cytol. 33, 157–252
Poste, G.; Alison, A.C. (1973): Membrane fusion. Biochim. Biophys. Acta 300, 421–463
Prives, J.; Shinitzky, M. (1977): Increased membrane fluidity precedes fusion of muscle cells. Nature 268, 761–763
Senda, M.; Takada, T.; Katagi, H.; Abe, S.; Yamada, Y. (1978): Effect of some polymers and temperature in fusion of plant protoplast. Abst. Ann. Meet. Agric. Chem. Soc. Japan, p. 19
Shinitzky, M.; Inbar, M. (1976): Microviscosity parameters and protein mobility in biological membranes. Biochim. Biophys. Acta 433, 133–149
Toister, Z.; Loyter, A. (1973): Ca+2-induced fusion of avian erythrocytes. Biochim. Biophys. Acta 241, 719–724
Yamada, Y.; Hara, Y.; Senda, M.; Nishihara, M.; Kito, M. (1979): Phospholipids of membranes of cultured cells and the products of protoplast fusion. Phytochemistry 18, 423–426
Yamada, Y.; Nakaminami, M. (1973): The isolation and fusion of protoplasts from alkaloid plant cells cultured in vitro. In: Protoplastes et fusion de cellules somatiques végétales. pp. 373–383. Paris: L'Institut National de la Recherche Agronomique
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Communicated by K. Tsunewaki
This work was supported by a grant from the Japanese Ministry of Education. (No. 310410 and No. 211115).
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Senda, M., Morikawa, H., Katagi, H. et al. Effect of temperature on membrane fluidity and protoplast fusion. Theoret. Appl. Genetics 57, 33–35 (1980). https://doi.org/10.1007/BF00276007
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DOI: https://doi.org/10.1007/BF00276007