Abstract
A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedlings were scored. Nine categories of transformants could be distinguished according to the number of loci into which T-DNA had inserted, and according to the effects of T-DNA integration on seed or seedling development. In most of the plants, T-DNA was inserted into a single site; others contained multiple independent copies of T-DNA. The number of T-DNA integration sites was found to be independent of whether a binary vector system or a cointegrate Ti plasmid had been used to obtain the transgenic plant. Loss of marker genes or marker gene expression from generation to generation appeared to be a quite frequent event. Plants which appeared to be insertional recessive embryo-lethal mutants did not exhibit this trait in the next generation.
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Abbreviations
- KanR :
-
kanamycin resistant
- KanS :
-
kanamycin sensitive
- NOP:
-
nopaline
- NOS:
-
nopaline synthase
- NPT II:
-
neomycin phosphotransferase II
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Communicated by J. Schell
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Heberle-Bors, E., Charvat, B., Thompson, D. et al. Genetic analysis of T-DNA insertions into the tobacco genome. Plant Cell Reports 7, 571–574 (1988). https://doi.org/10.1007/BF00272760
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DOI: https://doi.org/10.1007/BF00272760