Abstract
Polysomal poly(A)RNA was transcribed by avian myeloblastosis virus (AMV) reverse transcriptase and double-stranded complementary DNA (cDNA) was then synthesized and cloned in Escherichia coli C600 by (dG)-(dC) tailing in the PstI site of pBR322. A bank of mRNA sequences from maturating soybean seeds (average weight 200 mg) was obtained. 32P-cDNA based on mRNA from various organs of the plant (leaves, axes, seeds) was synthesized. The clones were hybridized with different kinds of 32P-cDNA on nitrocellulose filters. Approximately 13% of the clones were specific for the maturating stage, and more than a half the clones gave a positive result with all kinds of cDNA.
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Abbreviations
- AMV:
-
avian myeloblastosis virus
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Epishin, S.M., Kiselev, S.L. & Vinetski, Y.P. A colony hybridization study of mRNA in maturating soybean seeds. Plant Cell Reports 1, 267–269 (1982). https://doi.org/10.1007/BF00272636
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DOI: https://doi.org/10.1007/BF00272636