Abstract
The effect of the ethylene antagonists norbornadiene and silver nitrate and the ethylene precursor l-aminocyclopropane-l-carboxylic acid (ACC) on Zea mays plant regeneration was studied. A 12-fold increase in plant regeneration, as measured by number of plants obtained per gram fresh weight from callus cultures of maize inbreds Pa91 and H99, was obtained by 250 μM norbornadiene and 100 μM silver nitrate treatments. An increase in amout of nonregenerable tissue and a 68% decrease in plant regeneration were associated with callus treated with 1 mM ACC. Ethylene emanation from 1 mM ACC treated callus reached a maximum of 170 nl g−1 h−1 after 3 days compared to 7 nl g−1 h−1 for the control. The free proline content was up to 80% lower in 1 mM ACC treated callus grown for 30 days on medium with or without 12 mM proline, respectively, as compared to each control. These studies indicate that ethylene action inhibitors such as norbornadiene and silver nitrate can be used to increase plant regeneration efficiency from maize callus cultures.
Similar content being viewed by others
Abbreviations
- ACC:
-
l-aminocyclopropane-l-carboxylic acid
- gfw:
-
gram fresh weight
Literature Cited
Adams DO, Yang SF (1979) Proc. Natl. Acad. Sci. 76:170–174.
Apelbaum A, Goldlust A, Icekson I (1985) Plant Physiol. 79:635–640.
Armstrong C, Green CE (1985) Planta 164:207–214.
Burg SP, Burg EA (1968) Plant Physiol. 43:1069–1074.
Beyer, EM Jr (1976) Plant Physiol. 58:268–271.
Duncan DR, Williams ME, Zehr BE, Widholm JM (1985) Planta 165:322–332.
Duncan DR, Widholm JM (1987a) Plant Physiol. 83:703–708.
Duncan DR, Widholm JM (1987b) Plant Physiol. Supp. 83:35
Feirer RP, Mignon G, Litvay J (1984) Science 223:1433–1435.
Green CE, Phillips RL (1975) Crop Sci. 15:417–421.
Icekson I, Goldlust A, Apelbaum A (1985) J. Plant Physiol. 119:335–345.
Marissen N, van der Plas LHW, van der Maas R, Kanneworff WA (1987) Plant Science 51:43–49.
Perez-Bermudez P, Cornejo MJ, Segura J (1985) Plant Cell Rep. 4:188–190.
Purnhauser L, Medgyesy P, Czako M, Dix PJ, Marton L (1987) Plant Cell Rep. 6:1–4.
Reynolds T (1987) Am. J. Bot. 74:967–969.
Robinson KEP, Adams DO, Lee Ry (1987) Plant Cell Rep. 6:405–409.
Sauerbrey E, Grossmann K, Jung J (1987) J. Plant Physiol. 127:471–479.
Sisler EC, Yang SF (1984) Phytochem. 23:2765–2768.
Smith TA (1985) Ann. Rev. Plant Physiol. 36:117–143.
Songstad DD, Petracek PD, Sams CE, Conger BV (1985) Agronomy Abstracts p. 135.
van Aartrijk J, Blom-Barnhoorn GJ, Bruinsma J (1985) J. Plant Physiol. 117:401–410.
Wann SR, Johnson MA, Noland TL, Carlson JA (1987) Plant Cell Rep. 6:39–42.
Zobel RW, Roberts LW (1978) Can. J. Bot. 56:987–990.
Author information
Authors and Affiliations
Additional information
Communicated by E. D. Earle
Rights and permissions
About this article
Cite this article
Songstad, D.D., Duncan, D.R. & Widholm, J.M. Effect of l-aminocyclopropane-l-carboxylic acid, silver nitrate, and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Reports 7, 262–265 (1988). https://doi.org/10.1007/BF00272538
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00272538