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Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS

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Abstract

The reporter genes for Chloramphenicolacetyltransferase (CAT), Neomycinphosphotransferase-(NPT)-II and β-Glucuronidase (GUS) were compared in transient gene expression experiments in tobacco mesophyll protoplasts. For this purpose, nearly identical chimeric genes controlled by the CaMV 35 S promoter were constructed. The detection level of each system was determined yielding the following order of relative sensitivity: CAT<NPT II<GUS.

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Abbreviations

CAT:

Chloramphenicolacetyltransferase

NPT II:

Neomycinphosphotransferase

GUS:

β-Glucuronidase

CaMV:

Cauliflower Mosaic Virus

PEG:

Polyethylenglycol

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Abteilung Genetische Grundlagen der Pflanzenzüchtung, Egelspfad, D-5000, Köln 30, Federal Republic of Germany

    Reinhard Töpfer, Markus Pröls, Jozef Schell & Hans-Henning Steinbiß

Authors
  1. Reinhard Töpfer
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  2. Markus Pröls
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  3. Jozef Schell
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  4. Hans-Henning Steinbiß
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Communicated by H. Lörz

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Töpfer, R., Pröls, M., Schell, J. et al. Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS. Plant Cell Reports 7, 225–228 (1988). https://doi.org/10.1007/BF00272529

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  • DOI: https://doi.org/10.1007/BF00272529

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