Summary
We have analyzed the base composition of two heavy messenger RNAs synthesized by bacteriophage lambda early after induction:l 3 and r 2. The GC content of these two RNAs is very similar to the GC content of corresponding parts of λ DNA. This result confirms the approximate genetic location of l 3 and r 2 as determined by DNA-RNA hybridization experiments.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Calendar, R.: The regulation of phage development. Ann. Rev. Microbiol. 24, 241 (1970).
Champoux, J. J., Hogness, D. S.: The topography of the lambda chromosome: poly rG binding sites and base composition. Submitted to J. molec. Biol.
Gros, F., Kourilsky, P., Marcaud, L.: Pattern of gene transcription during the induction of bacteriophage lambda development: a possible model for the control of gene expression in a differentiating system. In Ciba Foundation Symposium on Homeostatic regulators, 107 (1969).
Herskowitz, I., Signer, E.: Control of transcription from the r strand of bacteriophage λ. Cold Spr. Harb. Symp. quant. Biol. 35, 355 (1970).
Karajanian, G.: Studies on the genetics of biotin-transducing, defective variants of bacteriophage λ. Virology 36, 30 (1968).
Kourilsky, P., Bourguignon, M.-F., Bouquet, M., Gros, F.: Early transcription controls after induction of prophage λ. Cold Spr. Harb. Symp. quant. Biol. 35, 305 (1970).
Kourilsky, P., Bourguignon, M.-F., Gros, F.: The kinetics of viral transcription after induction of prophage lambda. “The bacteriophage λ”. Proceedings of the Cold Spring Harbor λ meeting (in press).
—, Marcaud, L., Sheldrick, P., Luzzati, D., Gros, F.: Studies on the messenger RNA of bacteriophage λ, I. Various species synthesized early after induction of the prophage. Proc. nat. Acad. Sci. (Wash.) 61, 1013 (1968).
—, Portier, M.-M., Zamansky, M. H., Gros, F.: Etude des ARN messagers synthétisés après induction du prophage λ. Bull. Soc. Chim. biol. (Paris) 51, 1429 (1969).
Lozeron, H. A., Funderburgh, M. L., Szybalski, W.: Localization of 4,5 S RNA near the beginning of gene N in coliphage lambda. Fed. Proc. 30, 1315 Abs (1971).
Manly, K. F., Signer, E. R., Radding, C. M.: Non-essential functions of bacteriophage λ. Virology 37, 177 (1969).
Marcaud, L., Portier, M.-M., Kourilsky, P., Barrell, B. G., Gros, F.: A low molecular weight species of RNA synthesized early after induction of the prophage λ. J. molec. Biol. 57, 247 (1971).
Nijkamp, A. J. J., Bøvre, K., Szybalski, W.: Controls of rightward transcription in coliphage λ. J. molec. Biol. 54, 599 (1970).
Scherrer, K., Marcaud, L., Zadjela, F., Breckenridge, B., Gros, F.: Etude des RNA nucléaires et cytoplasmiques à marquage rapide dans les cellules érythropoïétiques aviaires différenciées. Bull. Soc. Chim. biol. (Paris) 48, 1037 (1966).
Skalka, A., Burgi, E., Hershey, A. D.: Segmental distribution of nucleotides in the DNA of bacteriophage λ. J. molec. Biol. 34, 1 (1968).
Author information
Authors and Affiliations
Additional information
Communicated by P. Starlinger
Rights and permissions
About this article
Cite this article
Marcaud, L., Portier, M.M., Kourilsky, P. et al. Genetic localization of two polycistronic messenger RNAs from bacteriophage lambda as deduced from their base compositions and annealing properties. Molec. Gen. Genet. 113, 355–358 (1971). https://doi.org/10.1007/BF00272335
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272335