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The involvement of DNA polymerase I in the postreplication repair of ultraviolet radiation-induced damage in Escherichia coli K-12

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Summary

A deficiency in DNA polymerase I increased the ultraviolet (UV) radiation sensitivity of a uvrA strain of Escherichia coli K-12 when plated on minimal growth medium. The slope of the survival curve for the uvrA polA strain was 2.0-times greater than that for the uvrA strain. The fluence-dependent yield of unrepaired deoxyribonucleic acid (DNA) parental-strand breaks following UV irradiation and incubation in minimal growth medium was similar in both strains. However, the fluence-dependent yield of unrepaired DNA daughter-strand gaps observed following UV irradiation was 1.8-fold greater in the uvrA polA strain than in the uvrA strain. These results suggest that DNA polymerase I is involved in the filling of at least some daughter-strand gaps during postreplication repair. Also, the uvrA polA strain was sensitized by a post-UV treatment with chloramphenicol (CAP) to a similar extent as was the uvrA strain, indicating that DNA polymerase I is not involved in the CAP-inhibitable pathway of postreplication repair.

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Author notes

  1. Thomas R. Barfknecht

    Present address: Department of Physiology, Harvard University school of Public Health, 02115, Boston, Massachusetts, USA

Authors and Affiliations

  1. Department of Radiology, Stanford University School of Medicine, 94305, Stanford, California, USA

    Thomas R. Barfknecht & Kendric C. Smith

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  1. Thomas R. Barfknecht
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  2. Kendric C. Smith
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Communicated by B.A. Bridges

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Barfknecht, T.R., Smith, K.C. The involvement of DNA polymerase I in the postreplication repair of ultraviolet radiation-induced damage in Escherichia coli K-12. Molec. Gen. Genet. 167, 37–41 (1978). https://doi.org/10.1007/BF00270319

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  • DOI: https://doi.org/10.1007/BF00270319

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