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Production of haploid plantlets in anther cultures of Albizzia lebbeck L.

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Abstract

Anthers of Albizzia lebbeck on B5 medium (BM) supplemented with kinetin (2 mg/l) and 2, 4-D (0.5 mg/l) showed callus initiation from microspores. Differentiation of embryoids and shoots was obtained on BM + BAP (1 mg/l) + IAA (0.5 mg/l) and of roots on BM. Root tip squashes of the regenerated plantlets showed the haploid chromosome number (n=13), confirming the microspore origin of the regenerants.

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Authors and Affiliations

  1. Department of Botany, University of Delhi, 110007, Delhi, India

    P. K. Gharyal, A. Rashid & S. C. Maheshwari

Authors
  1. P. K. Gharyal
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  2. A. Rashid
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  3. S. C. Maheshwari
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Communicated by J. Widholm

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Gharyal, P.K., Rashid, A. & Maheshwari, S.C. Production of haploid plantlets in anther cultures of Albizzia lebbeck L.. Plant Cell Reports 2, 308–309 (1983). https://doi.org/10.1007/BF00270188

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  • DOI: https://doi.org/10.1007/BF00270188

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