Abstract
The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-13C] proline was followed by 13C NMR and ninhydrin analyses of suspensions cultured in the presence of 0 or 260 mM NaCl. Uptake of 85 to 92% of the exogenous proline occurred within 72 h in all media. In 10 mM proline and no NaCl, cellular proline reached a maximm of 51.5 μmoles/g FW compared to 1.9 μmoles/g FW in suspensions not grown on proline. In medium containing 260 mM NaCl and proline, cellular proline reached 59–65 μmoles/g FW compared to 30–40 μmoles/g FW in controls grown without proline. The 13C-label in the proline-C1 was either retained in proline or disappeared, presumably released as carbon dioxide, by catabolism through the TCA cycle. Since no metabolite of 13C-proline was detected by NMR, proline was considered to be the molecule which inhibited the suspension culture growth.
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Abbreviations
- LS:
-
Linsmaier and Skoog medium
- FW:
-
fresh weight
- DW:
-
dry weight
- P5C:
-
Δ1-pyrroline-5-carboxylate
- TCA:
-
tricarboxylic acid cycle
- FID:
-
free-induction-decay
- NMR:
-
nuclear magnetic resonance spectroscopy
- T1 :
-
spin-lattice relaxation time
- NOE:
-
Nuclear Overhauser Effect.
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Rodriguez, M.M., Heyser, J.W. Growth inhibition by exogenous proline and its metabolism in saltgrass (Distichlis spicata) suspension cultures. Plant Cell Reports 7, 305–308 (1988). https://doi.org/10.1007/BF00269924
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DOI: https://doi.org/10.1007/BF00269924