Abstract
A glyphosate-tolerant variant of cultured tomato cells (Lycopersicon esculentum × L. peruvianum hybrid) was isolated via a single-step selection. Growth of the variant in suspension culture was essentially unaffected by 10 mM glyphosate, 100 times the concentration needed to significantly reduce the growth rate of wild type cells. When treated with glyphosate, variant cells accumulated much less shikimic acid than did the wild type cells. In analyses of 5-enolpyruvyl-shikimic acid 3-phosphate (EPSP) synthase activity in two separate experiments, the variant cells had 8 and 13 times higher specific activity than the wild type cells. The enzyme activities from the two types of cells were equally inhibited by glyphosate. These results suggest that the glyphosate tolerance of the variant results from overaccumulation of a glyphosate-sensitive EPSP synthase. Attempts to regenerate fertile plants from the variant cells were unsuccessful, but abnormal shoots were regenerated and callus from leaves of these shoots retained the tolerance to glyphosate.
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Amrhein N, Deus B, Gehrke P, Steinrucken HC (1980) Plant Physiol 66: 830–834
Amrhein N, Johanning D, Schab J, Schulz A (1983) FEBS Lett 157: 191–196
Baird DD, Upchurch RP, Homesley WB, Franz JE (1971) Proc North Central Weed Control Conf 26: 64–68
Boocock MR, Coggins JR (1983) FEBS Lett 154: 127–133
Bradford MM (1976) Anal Biochem 72: 248–254
Chaleff RS (1981) Genetics of higher plants: applications of cell culture. Cambridge Univ Press, Cambridge
Coma L, Facciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1985) Nature 317: 741–744
Comai L, Sen LC, Stalker DM (1983) Science 221: 370–371
Duncan K, Lewendon A, Coggins JR (1984) FEBS Lett 165: 121–127
Hogenboom GN (1972) Euphytica 21: 405–414
Hollander-Czytko H, Amrhein N (1983) Plant Sci Lett 29: 89–96
Ishikira N, Takeshima Y (1984) Plant Cell Physiol 25: 185–189
Jaworski EG (1972) J Agric Food Chem 20: 1195–1198
King J, Maretzki A (1983) Physiol Plant 58: 457–463
Lumsden J, Coggins JR (1977) Biochem J 161: 599–607
Meins F Jr (1983) Annu Rev Plant Physiol 34: 327–346
Nafziger ED, Widholm JM, Steinrucken HC, Killmer, JL (1984) Plant Physiol 76: 571–574.
Rogers SG, Brand LA, Holder SB, Sharps ES, Brackin MJ (1983) Appl Environ Microbiol 46: 37–43
Roisch U, Lingens F (1974) Angew Chem Intern Edit 13: 400
Rubin JL, Gaines CG, Jensen RA (1982) Plant Physiol 70: 833–838
Rubin JL, Gaines CG, Jensen RA (1984) Plant Physiol 75: 839–845
Schulz A, Sost D, Amrhein N (1984) Arch Microbiol 137: 121–123
Smart CC, Johänning D, Müller G, Amrhein N (1985) J Biol Chem 260: 16338–16346
Smith DDS, Coggins JR (1983) Biochem J 213: 405–415
Steinrucken HC, Amrhein N (1980) Biochem Biophys Res Commun 94: 1207–1212
Thomas BR, Pratt D (1982) Theor Appl Genet 63: 169–176
Weiss U, Mingioli ES (1956) J Am Chem Soc 78: 2894–2898
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Communicated by J.M. Widholm
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Smith, C.M., Pratt, D. & Thompson, G.A. Increased 5-enolpyruvylshikimic acid 3-phosphate synthase activity in a glyphosate-tolerant variant strain of tomato cells. Plant Cell Reports 5, 298–301 (1986). https://doi.org/10.1007/BF00269827
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DOI: https://doi.org/10.1007/BF00269827