Summary
Gene conversion was studied in a sample of 3869 unselected meiotic tetrads obtained from three diploids, respectively; heterozygous for a single ochre mutant, heteroallelic for a pair of ochre alleles, and heterozygous for an ochre specific suppressor. Although the genetic system were sufficiently sensitive to detect single base changes at the mutant codon level, none were found among 36 conversions (1+:3m) of the ochre mutants and 153 conversions (3S:1+) of the suppressor locus. These findings lead to the conclusion that the informational transfer in gene conversion occurred with complete fidelity. Gene conversion conserved and did not generate new genetic information. The error level of conversion was estimated as less than 10-2/N.P.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Case, M. E., Giles, N. H.: Allelic recombination in Neurospora. Tetrad analysis of a three point cross within the pan 2 locus. Genetics 49, 529–540 (1964).
Crick, F. H. C.: Codon-anticodon pairing: the wobble hypothesis. J. molec. Biol. 19, 548–555 (1966).
Demerec, M.: Mutable characters of Drosophila virilis I. Reddish-alpha body character. Genetics 13, 359–400 (1928).
Emerson, S.: Quantitative implication of the DNA repair model of gene conversion. Genetics 53, 475–485 (1966).
—: Fungal genetics. Ann. Rev. Genet. 1, 201–220 (1967).
—: Linkage and recombination at the chromosome level. In: Genetic organization, vol. I. New York: Academic Press, Inc. 1969.
—, Yu-Sun, C. C.: Gene conversion in the Pasadena strain of Ascobolus immersus. Genetics 55, 39–47 (1967).
Fogel, S., Hurst, D. D., Mortimer, R. K.: Gene conversion in unselected tetrads from multipoint crosses. Second Stadler Symp., Columbia, Mo. (May, 1970) (in press).
Gajewski, W., Paszewski, A., Dawidowicz, A., Dudzinski, B.: Postmeiotic segregation in locus ‘46’ of Ascobolus immersus. Genet. Res. (Camb.) 11, 311–317 (1968).
Gilmore, R. A.: Super-suppressors in Saccharomyces cerevisiae. Genetics 56, 641–658 (1967).
—, Stewart, J. W., Sherman, F.: Amino acid replacement resulting from super-suppression of a nonsense mutant of yeast. Biochim. biophys. Acta (Amst.) 161, 270–272 (1968).
Hawthorne, D. C.: The selection of nonsense suppressors in yeast. Mutation Res. 7, 187–197 (1969).
—: Identification of nonsense codons in yeast. J. molec. Biol. 43, 71–75 (1969).
— Mortimer, R. K.: Super-suppressors in yeast. Genetics 48, 617–620 (1963).
—: Genetic mapping of nonsense suppressors in yeast. Genetics 60, 735–742 (1968).
Holliday, R.: A mechanism for gene conversion in fungi. Genet. Res. 5, 282–304 (1964).
—: Genetic recombination in fungi. In: Replication and recombination of genetic material (ed. W. J. Peacock and R. D. Brock), p. 157–184. Canberra: Austral. Acad. Sci. 1968.
— Whitehouse, H. L. K.: The wrong way to think about gene conversion. Molec. Gen. Genetics: 107, 85–93 (1970).
Hurst, D. D., Fogel, S.: Mitotic recombination and heteroallelic repair in Saccharomyces cerevisiae. Genetics 50, 435–458 (1964).
Hurst, D. D., Fogel, S., Mortimer, R. K.: Conversion associated recombination in yeast. Proc. nat. Acad. Sci. (Wash.) (in press) (1970).
Magni, G. E.: The origin of spontaneous mutations during meiosis. Proc. nat. Acad. Sci. (Wash.) 50, 975–980 (1963).
—: Origin and nature of spontaneous mutation in meiotic organisms. J. cell. comp. Physiol. 64 (Suppl. 1), 165–172 (1964).
Magni, G. E., Mutation rate estimate.
Manney, T. R.: Evidence for chain termination by super-suppressible mutants in yeast. Genetics 60, 719–733 (1968).
Roman, H.: Sur les recombinaisons non-reciproques chez Saccharomyces cerevisiae et sur les problèms posís par ces phénomènes. Ann. Génét. 1, 11–17 (1958).
—: Genetic conversion in fungi, p. 209–227, Methodology in basic genetics, ed. W. J. Burdette. San Francisco: Holden Day 1963.
Sherman, F., Stewart, J. W., Parker, J. H., Putterman, G. J., Agrawal, B. B. L., Margoliash, E.: The relationship of gene structure and protein structure of iso-1-cytochrome c from yeast. S.E.B. Sympos. 24. The control of organelle development (in press) (1970).
Stadler, D. R., Towe, A.: Recombination of allelic cystsine mutants in Neurospora. Genetics 48, 1324–1344 (1963).
Stadler, D. R., Rossignol, J. L.: Intragenic recombination of ascospore color mutants in Ascobolus and its relationship to the segregation of outside markers. Genetics (in press) (1970).
Stahl, F.: One way to think about gene conversion. Genetics 61 (Suppl.), 1–13 (1969).
Stewart, J. W., Sherman, F.: Determination of nonsense codons in yeast by amino acid substitutions in iso-1-cytochrome c. Proc. XII Int. Cong. Genet., Tokyo 1, 45 (1968).
Whitehouse, H. L. K., Hastings, P. J.: The analysis of genetic recombination on the polaron hybrid DNA model. Genet. Res. 6, 27–92 (1965).
Winkler, H.: Die Konversion der Gene. Jena: Gustav Fischer 1930.
Zimmerman, F.: Enzyme studies on the products of mitotic gene conversion in Saccharomyces cerevisiae. Molec. Gen. Genetics 101, 171–184 (1968).
Author information
Authors and Affiliations
Additional information
Communicated by G. Magni
Rights and permissions
About this article
Cite this article
Fogel, S., Mortimer, R.K. Fidelity of meiotic gene conversion in yeast. Molec. Gen. Genet. 109, 177–185 (1970). https://doi.org/10.1007/BF00269654
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00269654