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Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) Czern

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Abstract

Isolated mesophyll protoplasts of Brassica juncea (L.) Czern., cv. RLM 514 upon culture in suitable growth medium, regenerated cell wall, underwent cell division and formed cellular colonies. Subsequent induction of embryoid (embryogenesis) and shoot bud (organogenesis) formations in such cell masses resulted in regeneration of 186 and 42 plantlets respectively.

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Abbreviations

NT:

Nagata and Takebe, 1971

B5:

Gamborg et al. 1968

KM:

Kao and Michayluk, 1975

GK2 :

Schenck and Hoffmann, 1978

MS:

Murashige and Skoog, 1962

BAP:

6-benzyladenine

2, 4-D:

2, 4-dichlorophenoxyacetic acid

NAA:

napthaleneacetic acid

GA3 :

gibberellic acid

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Authors and Affiliations

  1. Department of Botany, Bose Institute, 700 009, Calcutta, India

    Gouri Chatterjee, Samir R. Sikdar, Srabani Das & S. K. Sen

Authors
  1. Gouri Chatterjee
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  2. Samir R. Sikdar
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  3. Srabani Das
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  4. S. K. Sen
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Communicated by I. K. Vasil

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Chatterjee, G., Sikdar, S.R., Das, S. et al. Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) Czern. Plant Cell Reports 4, 245–247 (1985). https://doi.org/10.1007/BF00269368

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  • DOI: https://doi.org/10.1007/BF00269368

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