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Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens

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Abstract

In this paper we describe procedures for transforming micro colonies derived from mesophyll protoplasts of Petunia hybrida with Agrobacterium tumefaciens. The method is efficient, up to 70% of the colonies were transformed, and we used a similar method to transform cells from a suspension culture of haploid Nicotiana plumbaginifolia.

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Abbreviations

RH:

Relative humidity

MES:

2 (N - Morpholino) ethane sulphonic acid

NAA:

1 - Naphthylacetic acid

BAP:

6 - Benzyl amino purine

B5:

Gambourg's B5 medium (Gambourg 1970)

MS:

Murashige and Skoog Medium (1962)

PZ saline:

0.9% NaCl pH 7.0

2, 4-D:

2,4, Dichlorophenoxyacetic acid

Ty:

tryptone yeast

References

  • Barfield, D.G., S.J. Robinson, R. Shields (1985). Plant Cell Reports 4 104–107.

    Google Scholar 

  • Bopp, M. (1965). Nature 207 83–84.

    Google Scholar 

  • Braun, A.C. (1952). Growth 16 65–74.

    Google Scholar 

  • Caboche, M. (1980). Planta 149 7–18.

    Google Scholar 

  • Firoozabady, E. and D.W. Galbraith (1984). Plant Cell Tiss. and Organ Cult. 3 175–188.

    Google Scholar 

  • Fraley, R.T. et al (1983). Proc. Natl. Acad. Sci., U.S.A. 80 4803–4807.

    Google Scholar 

  • Fraley, R.T. et al (1984). Plant Mol. Biol. 3 371–378.

    Google Scholar 

  • Gambourg, O.L. (1970). Plant Physiol. 45 372.

    Google Scholar 

  • Giulotto, E. and N. Israel (1984). Biochem. Biophys. Res. Comm. 118 310–316.

    Google Scholar 

  • Hanold, D. (1983). Plant Sci. Lett 30 177–183.

    Google Scholar 

  • Jia, J.F., R.D. Shillito and I. Potrykus (1983). Z. Pflanzenphysiologie 112 1–6.

    Google Scholar 

  • Krens, F. (1983). Ph.D. Thesis. U. of Leiden.

  • Kupila-Ahvenniemi, S. and E. Therman (1971) Physiol. Plant 24 23–26.

    Google Scholar 

  • Lipetz, J. (1966). Cancer Res. 26 1597–1605.

    Google Scholar 

  • Marton, L., G.J. Wullems, L. Molendijk and R.A. Schilperoort (1979). Nature 277 129–131.

    Google Scholar 

  • Muller, A., T. Manzara and P.F. Lurquin. Biochem. Biophys. Res. Comm. 123 pp458–462.

  • Murashige, T. and Skoog, F. (1962). Physiol. Plant. 15 473–97.

    Google Scholar 

  • Nickell, L.G., W.D. Celmer (1965). Nature 206 302–304.

    Google Scholar 

  • Otten, L.A.B.M. and R.A. Schilperoort (1978). Biochem. Biophys. Acta 527 497–500.

    Google Scholar 

  • Pollock, K., D.G. Barfield and R. Shields (1983). Plant Cell Reports 2 36–39.

    Google Scholar 

  • Rennert, A., J. Kowalczyk and H. Pajkowska (1984). Acta Soc. Bot. Pol. 53 59–65.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Unilever Research, Colworth House, MK44 1LQ, Sharnbrook, Bedfordshire, UK

    Karen Pollock, Donna G. Barfield, Susan J. Robinson & Robert Shields

Authors
  1. Karen Pollock
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  2. Donna G. Barfield
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  3. Susan J. Robinson
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  4. Robert Shields
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Communicated by I. Potrykus

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Pollock, K., Barfield, D.G., Robinson, S.J. et al. Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens . Plant Cell Reports 4, 202–205 (1985). https://doi.org/10.1007/BF00269289

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  • DOI: https://doi.org/10.1007/BF00269289

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