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Fraction I protein analysis of parasexual hybrid plants Arabidopsis thaliana + Brassica campestris

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Abstract

The polypeptide composition of Fraction I protein (ribulose-1,5-bisphosphate carboxylase) prepared from leaves of two clones of the parasexual hybrid plant Arabidopsis thaliana + Brassica campestris as well as their parents was analyzed by isoelectric focusing. The protein in hybrid plants contained a heterogenous population of small subunits resulting from the expression of both Arbabidopsis and Brassica nuclear genes, whereas the large subunit polypeptides, and hence the functional chloroplast DNA, were from the Brassica parent.

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Authors and Affiliations

  1. Laboratory of Cytophysiology and Plant Cell Engineering, Institute of Botany of the Academy of Sciences of the Ukrainian SSR, Repina 2, 252601, Kiev, USSR

    I. K. Komarnitsky & Yu.Yu. Gleba

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  1. I. K. Komarnitsky
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  2. Yu.Yu. Gleba
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Komarnitsky, I.K., Gleba, Y. Fraction I protein analysis of parasexual hybrid plants Arabidopsis thaliana + Brassica campestris . Plant Cell Reports 1, 67–68 (1981). https://doi.org/10.1007/BF00269275

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  • DOI: https://doi.org/10.1007/BF00269275

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