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Introduction of Escherichia coli cells and spheroplasts into Vinca protoplasts

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Abstract

In the presence of 10% polyvinyl alcohol (PVA), Escherichia coli cells or spheroplasts can be easily introduced into Vinca protoplasts by endocytosis. Uptake proceeded quite rapidly; bacterial cells or spheroplasts were found within the cytoplasm of Vinca protoplasts after 10 min of incubation with PVA.

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Authors and Affiliations

  1. Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya

    C. Matsui & N. Tanaka

  2. Department of Pure and Applied Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, Japan

    S. Hasezawa & K. Syono

Authors
  1. C. Matsui
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  2. S. Hasezawa
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  3. N. Tanaka
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  4. K. Syono
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Matsui, C., Hasezawa, S., Tanaka, N. et al. Introduction of Escherichia coli cells and spheroplasts into Vinca protoplasts. Plant Cell Reports 2, 30–32 (1983). https://doi.org/10.1007/BF00269230

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  • DOI: https://doi.org/10.1007/BF00269230

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