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Shoot and root organogenesis of Camellia sasanqua

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Abstract

In vitro-derived shoot tips, (10 mm) taken from primary cultures of Camellia sasanqua L., were evaluated for organogenesis when cultured on a half-strength MS medium supplemented with various concentrations of NAA, IBA, BA and GA3. Maximum shoot proliferation and growth for juvenile and mature tissue was obtained when 0.54 μM NAA, 8.8 μM BA plus 14.4 to 28.9 μM GA3 was added to the culture media, with a pH between 4.5 and 5.0. In vitro-derived shoots (20 mm) from mature C. sasanqua ‘Day Dream’ and juvenile C. sasanqua cultures initiated roots in vitro after immersion in 2.5 mM IBA for 30 min. Sixty percent of the mature shoots and 90% of the juvenile shoots initiated roots within 3 weeks of treatment with IBA.

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Abbreviations

MS:

Murashige and Skoog (1962)

IBA:

lH-indole-3-butanoic acid

BA:

N-(phenyl-methyl)-lH-purine-6-amine

GA3 :

gibberellic acid

kinetin:

N-(Z-furanyl-methyl)-lH-purine-6-amine

NAA:

l-naphthaleneacetic acid

L:

Linear

Q:

Quadratic

References

  • Bennett WY, Scherbert P (1982) Camellia J. 37 (1):12–15

    Google Scholar 

  • Broome OC, Zimmerman RH (1978) HortScience 13:151–153

    Google Scholar 

  • Carlisi JA (1985) Factors affecting in vitro propagation of Camellia. M. S. Thesis, Louisiana State University

  • George EF, Sherrington PD (1984) Plant Propagation by Tissue Culture. Exgetics, Ltd. Eversley, England

    Google Scholar 

  • Jones OP, Hopgood ME, O'Farrell D (1977) J. Hort. Sci. 52:235–238

    Google Scholar 

  • Lane WD (1978) Plant Sci. Lett. 13:281–285.

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497

    Google Scholar 

  • Samartin A, Vieitez AM, Vieitez E (1984) HortScience 19:225–226

    Google Scholar 

  • Schnabelrauch, LS and Sink KC (1979) HortScience 14(5):607–608

    Google Scholar 

  • Sharma AK, Prasad RN, Chaturvedi HC (1981) Plant Cell, Tissue and Organ Culture 1:33–38.

    Google Scholar 

  • Takayama S, Misawa M (1979) Physiol Plant 46:184–190

    Google Scholar 

  • Venverloo C (1978) In Thorpe, TA (ed.) 4th Inter. Congr. Plant Tissue and Cell Culture, Univ. of Calgary, Canada p. 28

    Google Scholar 

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Authors and Affiliations

  1. Department of Horticulture, 137 Julian C. Miller Hall, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803, Baton Rouge, LA, USA

    Kenneth C. Torres & Jacqueline A. Carlisi

Authors
  1. Kenneth C. Torres
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  2. Jacqueline A. Carlisi
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Communicated by G. C. Phillips

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Torres, K.C., Carlisi, J.A. Shoot and root organogenesis of Camellia sasanqua . Plant Cell Reports 5, 381–384 (1986). https://doi.org/10.1007/BF00268607

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  • DOI: https://doi.org/10.1007/BF00268607

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