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Molecular interactions of ribosomal components

II. Site-specific complex formation between 30S proteins and ribosomal RNA

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Summary

Five of the 30S ribosomal proteins from E. coli were tested for their ability to bind to 16S ribosomal RNA. Only one of these, S15, can form a complex with the RNA. Quantitative measurements as well as competition experiments show that the RNA binding site for the attachment of S15 is specific for this protein.

These experiments complete our analysis of all 21 of the 30S ribosomal proteins. Five of these have now been shown to form a site-specific complex with 16S RNA. These are S4, S7, S8, S15 and S20. The relationship of these data to the assembly and structure of the ribosome are discussed.

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Authors and Affiliations

  1. Department of Zoology, University of Wisconsin, Madison, Wisconsin, USA

    H. W. Schaup, M. Green & C. G. Kurland

  2. The Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden

    H. W. Schaup, M. Green & C. G. Kurland

Authors
  1. H. W. Schaup
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  2. M. Green
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  3. C. G. Kurland
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Communicated by H. G. Wittmann

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Schaup, H.W., Green, M. & Kurland, C.G. Molecular interactions of ribosomal components. Molec. Gen. Genet. 112, 1–8 (1971). https://doi.org/10.1007/BF00266926

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  • DOI: https://doi.org/10.1007/BF00266926

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